Immortal avian cell line to grow avian and animal viruses to produce vaccines

A method for propagating an avian virus, comprising:

• (a) providing a cell line generated from chicken embryo cells that have been treated with a chemical mutagen to create an immortal cell line said cell line being virus free;
• (b) infecting said immortal cell line with an avian virus selected from the group of virus families consisting of Adenoviradae, Birnaviradae, Coronaviradae, Herpesviradae Orthomyxoviradae, Picornaviradae, Poxviradae, Reoviradae, and Polyomaviradae;
• (c) cultivating the infected immortal cell line to produce the immortal cell line infected with the avian virus;
• and (d) removing the virus from the immortal cell line.

A method for providing an avian vaccine, comprising:

• (a) providing a cell line generated from chicken embryo cells that have been treated with a chemical mutagen to create an immortal cell line;
• (b) infecting said cell line with a virus;
• (c) cultivating said infected cell line to produce the virus;
• (d) harvesting the virus from the cultivated cell line;
• and (e) subjecting the harvested virus to at least one of the following treatments:
• (i) inactivating the virus, (ii) adding a pharmaceutically acceptable carrier or diluent, (iii) adding an adjuvant, (iv) adding a stabilizer, (v) lyophilizing, wherein the vaccine induces protective immunity in avians.

A method for propagating swine influenza virus, comprising:

• (a) providing a cell line generated from chicken embryo cells that have been treated with a chemical mutagen to create an immortal cell line said cell line being virus free;
• (b) infecting said cell line with swine influenza virus;
• (c) cultivating the infected cell line to produce the swine influenza virus;
• and removing the virus from the cell line.

A method for preparing a swine influenza vaccine, comprising:

• (a) providing a cell line generated from chicken embryo cells that have been treated with a chemical mutagen to create an immortal cell line;
• (b) infecting said cell line with the virus;
• (c) cultivating said infected cell line to produce the virus;
• (d) harvesting the virus from the cultivated cell line;
• and (e) subjecting the harvested virus to at least one of the following treatments:
• (i) inactivating the virus, (ii) adding a pharmaceutically acceptable carrier or diluent, (iii) adding an adjuvant, (iv) lyophilizing, wherein the vaccine induces protective immunity in swine.

A method for propagating a virus that replicates in embryonated eggs, comprising:

• (a) providing a cell line generated from chicken embryo cells that have been treated with a chemical mutagen to create an immortal cell line said cell line being virus free;
• (b) infecting said cell line with a virus selected from the group of virus families consisting of Adenoviradae, Birnaviradae, Coronaviradae, Herpesviradae Orthomyxoviradae, Picornaviradae, Poxviradae, Reoviradae, and Polyomaviradae;
• (c) cultivating the infected cell line to produce an immortal cell line infected with the virus;
• and (d) removing the virus from the cell line.

A method for preparing a vaccine from a virus that replicates in embryonated eggs, comprising:

• (a) providing a cell line generated from chicken embryo cells that have been treated with a chemical mutagen to create an immortal cell line;
• (b) infecting said cell line with the virus;
• (c) cultivating said infected cell line to produce the virus;
• (d) harvesting the virus from the cultivated cell line;
• and (e) subjecting the harvested virus to at least one of the following treatments:
• (i) inactivating the virus, (ii) adding a pharmaceutically acceptable carrier or diluent, (iii) adding an adjuvant, (iv) lyophilizing, wherein the vaccine induces protective immunity in a host animal.

A method for preparing a vaccine from a virus that replicates in chicken embryo fibroblasts, comprising:

• (a) providing a cell line generated from chicken embryo cells that have been treated with a chemical mutagen to create an immortal cell line;
• (b) infecting said cell line with the virus;
• (c) cultivating said infected cell line to produce the virus;
• (d) harvesting the virus from the cultivated cell line;
• and (e) subjecting the harvested virus to at least one of the following treatments:
• (i) inactivating the virus, (ii) adding a pharmaceutically acceptable carrier or diluent, (iii) adding an adjuvant, (iv) lyophilizing, wherein the vaccine induces protective immunity in a host animal.

A method for propagating a virus that has been modified to replicate in chicken embryo fibroblasts, comprising:

• (a) providing a cell line generated from chicken embryo cells that have been treated with a chemical mutagen to create an immortal cell line said cell line being virus free;
• (b) infecting said cell line with the virus;
• (c) cultivating the infected cell line to produce the virus;
• and (d) removing the virus from the chicken embryo cell line.

A method for preparing a vaccine from a virus that has been modified to replicate in chicken embryo fibroblasts, comprising:

• (a) providing a cell line generated from chicken embryo cells that have been treated with a chemical mutagen to create an immortal cell line;
• (b) infecting said cell line with the virus;
• (c) cultivating said infected cell line to produce the virus;
• (d) harvesting the virus from the cultivated cell line;
• and (e) subjecting the harvested virus to at least one of the following treatments:
• (i) inactivating the virus, (ii) adding a pharmaceutically acceptable carrier or diluent, (iii) adding an adjuvant, (iv) lyophilizing, wherein the vaccine induces protective immunity in a host animal.

A method for identifying a virus, comprising:

• (a) providing a cell line generated from chicken embryo cells that have been treated with a chemical mutagen to create an immortal cell line said cell line being virus free;
• (b) infecting said cell line with the virus;
• (c) cultivating the infected cell line to produce the virus;
• (d) reacting said infected cell line with an antibody specific for the virus;
• and (e) visualizing infected cells reacted with the antibody.