Device and methods for multigradient directional cooling and warming of biological samples
AssigneeInterface Multigrad Technology
InventorAmir Arav
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Abstract
A device for controlled freezing and warming of a biological sample, and freezing and thawing protocols for which the device is well suited. The device establishes a laterally varying thermal gradient and provides a mechanism for moving the sample along the thermal gradient at a controlled rate of speed. The sample is moved along the thermal gradient at a rate of speed that provides a variable cooling rate or a variable warming rate in accordance with the appropriate protocol. The device also allows continuous seeding of the sample through the freezing process at the exact freezing point of the solution. Real time monitoring and video imaging of the freezing process enable fine tuning of the thermodynamic parameters for improved control.
Claims16
A device for freezing a biological sample, comprising:
- (a) a track;
- (b) refrigeration means for imposing a laterally variable temperature gradient along said track, said refrigeration means including a plurality of thermally conductive blocks substantially enclosing said track;
- and
- (c) a mechanism for moving the biological sample along said track.
A device for freezing a biological sample, comprising:
- (a) a track having an exit;
- (b) refrigeration means for imposing a laterally variable temperature gradient along said track;
- (c) a mechanism for moving the biological sample along said track;
- and
- (d) a container of a cryogenic fluid, positioned to receive the sample as the sample emerges from said exit.
Dependencies are inferred from the claim text.