Antigen-carrying microparticles and their use in the indication of humoral or cellular responses
AssigneeInstitut Pasteur
InventorsChristine Gengoux-Sedlik, Claude LeClerc
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Abstract
The invention concerns the use, in the induction of an immune response, of a synthetic microparticle polymer carrying on the surface one or more covalently bonded proteins capable of carrying one or more epitopes, the densities of the protein(s) on the surface of the microparticles, and their molecular weights, being adjusted so as to direct the immune response to the induction of a humoral and cellular response or to the induction of a largely cellular response. Said microparticles have an average diameter of approximately 0.25 to 1.5 .mu.m.
Claims27
A method of inducing an immune response in warm-blooded animals comprising administering to warm-blooded animals an immune response inducing amount of synthetic biocompatible microparticles having an average diameter between about 0.25 .mu.m and 1.5 .mu.m and carrying on their surface at least one covalently bonded protein, each carrying at least one epitope to induce an humoral or cellular immune response, the density of the protein(s) on the microparticle surfaces being adjusted to comprise between 10.sup.4 to 5.10.sup.5 molecules per .mu.m.sup.2 and to direct the said immune response towards the induction of cellular and/or humoral response.
Process for the production of microparticles whose immune response is either mainly humoral or mainly cellular, said process being characterized in that at least one protein carrying one or more epitopes or peptides containing T or B epitopes alone or a combination of the two are covalently fixed to synthetic polymer microparticles, the density of the protein fixed to the surface being varied according to the humoral or cellular response required.
Synthetic biocompatible microparticles having an average diameter between about 0.25 .mu.m and 1.5 .mu.m and carrying on their surface at least one covalently bonded protein, each carrying at least one epitope to induce an humoral or cellular immune response, the density of the protein(s) on the microparticle surfaces being adjusted to comprise between 10.sup.4 to 5.10.sup.5 molecules per .mu.m.sup.2 and to direct the said immune response towards the induction of cellular and/or humoral response.
Dependencies are inferred from the claim text.