US 5,866,140 AGranted

Type I surface antigen associated with staphylococcus epidermidis

AssigneeNabi

InventorsAli Ibrahim Fattom, Walter W. Karakawa, deceased, D. Craig Wright

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Abstract

A process is disclosed for culturing clinical Staphylococcus epidermidis cells that reproducibly enables identification of a limited number of predominant serotypes. Two predominant serotypes common to most clinical cases of S. epidermidis have been identified and are denoted Type I and Type II. A particular polysaccharide surface antigen is associated with each of the Type I and Type II serotypes. The surface antigens can be used to provide active and passive immunization against S. epidermidis infection and to produce a hyperimmune immunoglobulin or antibodies for treatment of S. epidermidis infection.

Claims10

Dependent claims

A vaccine comprising a sterile, pharmaceutically-acceptable carrier and a composition consisting essentially of a polysaccharide antigen that is obtained by a process comprising steps of:

growing cells of a Type I isolate of S. epidermidis that agglutinates antisera to ATCC 55254;
extracting polysaccharide antigen from said cells to produce a crude extract of Type I polysaccharide antigen;
purifying said crude extract to produce purified antigen that contains less than 1% protein;
loading said purified antigen on a separatory column and eluting it with a NaCl gradient;
and
identifying fractions containing Type I polysaccharide antigen using antibodies specific to a Type I isolate.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application: 08/361,821Filed 22 Dec 1994 · continuation
Priority: 22 Nov 1991
Granted: 2 Feb 1999
Term ends: Not estimatedThe source records don’t support an estimate.

Classification10 codes

A61K 39/085
Bacterial antigens: staphylococcus
A61P 31/04
Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics: antibacterial agents
A61P 37/04
Immunomodulators: immunostimulants
C07K 16/1271
Gram-positive bacteria: micrococcaceae ; Staphylococcaceae , e.g. Staphylococcus

Family

Serial796,252Parent of this continuation · filed 22 Nov 1991
Serial142,117Parent of this continuation · filed 28 Oct 1993
US 5,866,140 AThis patent

From the US filing history. Foreign family members aren’t listed.

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