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US 5,858,790 AGranted
Hematology reference control and method of preparation
AssigneeAbbott Laboratories
InventorsYoung Ran Kim, Jean Emiko Kihara
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Abstract
Hematology reference control cells and method of manufacture. The invention relates to the methods of preparing stable white blood cell ("WBC") and nucleated red blood cell ("NRBC") fractions and the hematology control reagents containing such stablized cells for primary use on a multi-angle light scatter based hematology instrument.
Claims4
A hematology control suspension comprising:
- a white blood cell ("WBC") fraction consisting essentially of human WBC wherein the human WBC possess predetermined cellular multi-angle light scattering characteristics similar to the cellular multi-angle light scattering characteristics of WBC of interest and which have been subjected to a lysing and fixing process that fixes the human WBC prior to destruction of the multi-angle light scattering characteristics of the human WBC so that subpopulations of WBC may be differentiated solely by the use of a multi-angle light scatter hematology analyzer.
A hematology control suspension comprising:
- a white blood cell ("WBC") fraction consisting essentially of mammalian WBC wherein the mammalian WBC possess predetermined cellular multi-angle light scattering characteristics similar to the cellular multi-angle light scattering characteristics of WBC interest and which have been subjected to a lysing and fixing process that fixes the mammalian WBC prior to destruction of the multi-angle light scattering characteristics of the mammalian WBC;
- the hematology control suspension further comprising a nucleated red blood cell ("NRBC") fraction wherein cells of the NRBC fraction are selected from the group consisting of avian erythrocytes, fish erythrocytes and mammalian lymphocytes, wherein nuclei of the cells in the NRBC fraction possess predetermined cellular multi-angle light scattering characteristics similar to the cellular multi-angle light scattering characteristics of nuclei in NRBC cells of interest and which have been subjected to a lysing and fixing process that lyses cytoplasm and fixes nuclei of the cells in the NRBC fraction prior to destruction of the desired light scattering characteristics of the nuclei.
A hematology whole blood control suspension comprising:
- a stabilized red blood cell ("RBC") fraction;
- a stabilized platelet ("PLT") fraction;
- a stabilized white blood cell ("WBC") fraction;
- and
- a resuspension and storage medium,
- wherein the WBC fraction consists essentially of human WBC wherein the human WBC possess predetermined cellular multi-angle light scattering characteristics similar to the cellular multi-angle light scattering characteristics of WBC of interest and which have been subjected to a lysing and fixing process that fixes the human WBC prior to destruction of the multi-angle light scattering characteristics of the human WBC so that the subpopulations of WBC may be differentiated solely by the use of a multi-angle light scatter hematology analyzer.
3 more elements
A hematology whole blood control suspension comprising:
- a stabilized red blood cell ("RBC") fraction;
- a stabilized platelet ("PLT") fraction;
- a stabilized white blood cell ("WBC") fraction;
- and
- a resuspension and storage medium,
- wherein the WBC fraction consists essentially of mammalian WBC wherein the mammalian WBC possess predetermined cellular multi-angle light scattering characteristics similar to the cellular multi-angle light scattering characteristics of WBC of interest and which have been subjected to a lysing and fixing process that fixes the mammalian WBC prior to destruction of the multi-angle light scattering characteristics of the mammalian WBC;
- the hematology control suspension further comprising a nucleated red blood cell ("NRBC") fraction wherein cells of the NRBC fraction are selected from the group consisting of avian erythrocytes, fish erythrocytes and mammalian lymphocytes, wherein nuclei of the cells in the NRBC fraction possess predetermined cellular multi-angle light scattering characteristics similar to the cellular multi-angle light scattering characteristics of nuclei in NRBC cells of interest and which have been subjected to a lysing and fixing process that lyses cytoplasm and fixes nuclei of the cells in the NRBC fraction prior to the destruction of the light scattering characteristics of the nuclei.
4 more elements
Dependencies are inferred from the claim text.