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US 5,858,762 AGranted
Plasmid for transformation of root nodule bacteria
AssigneeWisconsin Alumni Research Foundation
InventorEric W. Triplett
Life of this patent
Estimating the term…
Abstract
A plasmid for use in root nodule bacteria is described which is intended both to increase yield of inoculated legume plants inoculated with the bacteria as well as aiding in the competitiveness of the bacteria with native strains. The plasmid include a hydrogen uptake element which aids in energy efficient nitrogen fixation by the plant bacteria symbiotic pair as well as a toxin and resistance element intended to aid in the competitive fitness of the inoculant bacterial strain.
Claims9
A plasmid comprising
- a genetic element which expresses uptake hydrogenase activity in a root nodule bacterial strain,
- a genetic element which expresses trifolitoxin production and resistance in a root nodule bacterial strain,
- a partitioning element which has the effect of ensuring that the plasmid is inherited by viable daughter cells following asexual reproduction of a root nodule bacteria harboring the plasmid, and
- an origin of replication effective in root nodule bacteria.
1 more element
Plasmid pHUTFXPAR.
Dependencies are inferred from the claim text.