US 5,858,746 A · Gels for encapsulation of biological materials

Abstract

Water soluble macromers are modified by addition of free radical polymerizable groups, such as those containing a carbon-carbon double or triple bond, which can be polymerized under mild conditions to encapsulate tissues, cells, or biologically active materials. The polymeric materials are particularly useful as tissue adhesives, coatings for tissue lumens including blood vessels, coatings for cells such as islets of Langerhans, coatings, plugs, supports or substrates for contact with biological materials such as the body, and as drug delivery devices for biologically active molecules.

Claims31

Dependent claims

A method for encapsulating, sealing, plugging, or supporting of mammalian cells, cell aggregates, or cell tissue comprising:

a) applying (i) a water soluble biocompatible macromer comprising at least two free radical-polymerizable substituents, wherein the macromer is non-toxic and has a molecular weight of at least 400, and (ii) a non-toxic free radical polymerization initiator selected from the group consisting of visible light or long wavelength ultraviolet light-activatable free radical initiators, thermal activatable free radical initiators, benzoyl peroxide, potassium persulfate and ammonium persulfate;
to (iii) mammalian cells, cell aggregates, or cell tissue;
and
b) exposing the mixture to an activating agent to cause polymerization of the macromers.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

08/377,911Filed 25 Jan 1995 · continuation

Priority

20 Apr 1992

Granted

12 Jan 1999

Term ends

Not estimatedThe source records don’t support an estimate.

Classification57 codes

C12N 11/04
Enzymes or microbial cells immobilised on or in an organic carrier: entrapped within the carrier, e.g. gel or hollow fibres
A61K 9/1635
Organic macromolecular compounds: obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyvinyl pyrrolidone, polyacrylates
A61K 9/1647
Obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyethylene glycol, poloxamers: polyesters, e.g. poly
A61K 9/5026
Organic macromolecular compounds: obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyvinyl pyrrolidone, polyacrylates

Family

Serial870,540Parent of this continuation-in-part · filed 20 Apr 1992

Serial958,870Parent of this continuation-in-part · filed 7 Oct 1992

Serial24,657Parent of this continuation · filed 1 Mar 1993

US 5,573,934 AParent of this continuation

US 5,529,914 AParent of this continuation-in-part

US 5,858,746 AThis patent

From the US filing history. Foreign family members aren’t listed.

Gels for encapsulation of biological materials

Life of this patent

Gels for encapsulation of biological materials

Life of this patent

Abstract

Claims31