Method for identifying compounds for inhibition of cancerous lesions
AssigneeCell Pathways, Inc.
InventorsGary A. Piazza, Rifat Pamukcu, W. Joseph Thompson
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Abstract
This invention provides a method to identify compounds potentially useful for the treatment of neoplasia in mammals. The phosphodiesterase inhibitory activity of a compound is determined along with COX inhibitory activity. Growth inhibitory and apoptosis inducing effects on cultured tumor cells are also determined. Compounds that exhibit phosphodiesterase inhibiton, growth inhibition and apoptosis induction, but not substantial prostaglandin inhibitory activity, are desirable for the treatment of neoplasia.
Claims26
A method for identifying a compound with potential for treating neoplasia, comprising
- determining cyclooxygenase (COX) inhibitory activity of the compound;
- and
- determining the PDE-5 inhibition activity of said compound;
- wherein low COX inhibitory activity and high inhibition of PDE-5 activity identifies that the compound has potential for treating neoplasia.
A method of selecting compounds potentially useful for treating of neoplasia, comprising
- determining the neoplastic growth inhibitory activity of the compound;
- determining the PDE-5 inhibition activity of the compound;
- and
- selecting compounds for treating neoplasia that exhibit growth inhibitory activity and PDE-5 inhibitory activity.
A method for identifying compounds potentially useful for administering to patients in need of treatment for neoplasia, comprising the steps of:
- determining the COX inhibitory activity of the compounds;
- determining the phosphodiesterase Type V inhibitors activity of the compounds;
- and
- identifying those compounds for potential use in treating neoplasia in patients in need thereof if the compounds exhibit phosphodiesterase Type V inhibitory activity and have low COX inhibitory activity.
A method for selecting compounds with potential for treating neoplasia comprising determining the phosphodiesterase Type V inhibitory activity of said compounds and selecting compounds with inhibitory activity.
A method for identifying a compound with potential for treating neoplasia, comprising:
- determining the cyclooxygenase-I inhibiting activity of the compound;
- and
- determining the phosphodiesterase type 5 inhibiting activity of the compound;
- wherein low cyclooxygenase-I inhibiting activity and high inhibition of phosphodiesterase type 5 activity identifies that a compound has potential for treating neoplasia.
A method for identifying a compound with potential for treating neoplasia, comprising:
- treating neoplastic cells with the compound;
- determining the intracellular amount of cGMP in the treated and untreated cells;
- determining the intracellular amount of cAMP in the treated and untreated cells;
- wherein an increase in the ratio of cGMP/cAMP in the treated cells compared to the ratio of cGMP/cAMP in untreated neoplastic cells identifies that the compound has potential for treating neoplasia.
A method for identifying a compound with potential for treating neoplasia, comprising:
- selecting a compound with PDE-5 inhibiting activity;
- and
- evaluating said neoplastic cell growth inhibiting activity of the compound wherein a compound that has PDE-5 inhibiting activity and neoplastic cell growth inhibiting activity has the potential to inhibit neoplasia without substantially inhibiting the growth of normal cells.
Dependencies are inferred from the claim text.