US 5,858,688 A · Gro genes, proteins, and uses thereof

Abstract

Two novel cytokines which are involved in an inflammatory response (inflammatory cytokines) are disclosed. The inflammatory cytokines have been isolated from activated peripheral blood, preferably monocytes adhered to plastic; or peripheral blood leukocytes induced with calcium ionophore and mezerin. The two cytokines are Gro .beta. and .gamma., and their cDNA and amino acid sequences are disclosed. The expression of the genes in various cells is presented. Assay and diagnostic utilities using the cytokines; recombinant materials and procedures; purification procedures; and monoclonal antibodies to the cytokines are also shown.

Claims1

Dependent claims

A method for determining whether a chemical has inflammatory activity, comprising the steps of:

measuring Gro .beta. or .gamma. level in inflammatory responsive cells which have been exposed to the chemical, and comparing the Gro .beta. or .gamma. level to that of cells which have not been exposed to the chemical.

Dependencies are inferred from the claim text.

Facts

Application

08/474,362Filed 7 Jun 1995 · division

Priority

28 Sep 1990

Granted

12 Jan 1999

Term ends

Not estimatedThe source records don’t support an estimate.

Classification12 codes

C12Q 1/6883
Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes: for diseases caused by alterations of genetic material
A61K 49/0004
Preparations for testing in vivo: screening or testing of compounds for diagnosis of disorders, assessment of conditions, e.g. renal clearance,…
A61K 49/0008
Screening or testing of compounds for diagnosis of disorders, assessment of conditions, e.g. renal clearance, gastric emptying, testing for…:…
A61K 51/08
Organic compounds: peptides, e.g. proteins , carriers being peptides, polyamino acids, proteins

Gro genes, proteins, and uses thereof

Life of this patent

Gro genes, proteins, and uses thereof

Life of this patent

Abstract

Claims1