Prostate specific antigen from benign prostatic hyperplasia and its use in diagnosis
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsThomas A. Stamey, Zuxiong Chen, John E. McNeal
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Abstract
Benign prostatic hyperplasia-prostate specific antigen (BPH-PSA) and methods for its preparation are provided. Also provided are antibodies specific for BPH-PSA, and methods of their use in immunoassays for detecting the presence of BPH-PSA in a sample. The subject immunoassays find use in diagnosing the conditions associated with elevated serum PSA levels, such as prostate cancer and BPH.
Claims21
A method of obtaining a preparation of Benign Prostatic Hyperplasia Prostate Specific Antigen (BPH-PSA), the method comprising:
- obtaining a fluid comprising BPH-PSA from BPH nodules;
- and
- enriching the fluid with respect to said BPH-PSA.
A method for purifying BPH-PSA from a naturally occurring fluid comprising BPH-PSA, the method comprising:
- harvesting BPH nodules from a human male patient having benign prostatic hyperplasia;
- piercing the nodules to release BPH nodule fluid present in the nodules;
- collecting the released BPH-nodule fluid;
- subjecting the BPH-nodule fluid to a two step purification procedure comprising gel chromatography and ion-exchange chromatography;
- whereby the BPH-PSA is purified.
BPH-PSA having an enzymatic activity that differs from seminal fluid PSA.
Dependencies are inferred from the claim text.