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US 5,858,676 AGranted
Electrochemiluminescence of rare earth metal chelates
AssigneeIGEN International, Inc.
InventorsHongjun Yang, Nicholas Cairns
Life of this patent
Estimating the term…
Abstract
Luminescent chemical reagents that include complexes of rare earth metals with ligands such as aromatic heterocyclic nitrogen-containing compounds and semi-aromatic oxygen-containing compounds are used to detect small quantities of complex substances such as pharmaceuticals, metabolites, and microorganisms in complex sample mixtures.
Claims15
A method of determining the presence of a chemical moiety, said chemical moiety having the formula
- MPL.sup.1 L.sup.2 -(-link-)-!.sub.t B
- wherein M is a lanthanide;
- P is a polydentate ligand of M;
- L.sup.1 and L.sup.2 are ligands of M, each of which may be a substance covalently bound to one or more of P, L.sup.1 or L.sup.2 through one or more covalent bond linkages, said linkages designated as (-link-) and being covalent bonds linking B with at least one of P, L.sup.1 or L.sup.2 ;
- t is an integer equal to or greater than 1;
- B is a biological substance, a synthetic substance which is capable of competing with a biological substance in a competitive binding reaction with a complementary material, or a non-biological substance;
- P, L.sup.1 or L.sup.2, and B are of such number that the total number of bonds to M provided by the ligands of M equals the coordination number of m;
- and
- P, L.sup.1 or L.sup.2, and B are of such composition that the chemical moiety can be induced to repeatedly emit electromagnetic radiation;
- which method comprises
- (a) forming a reagent mixture containing the chemical moiety, or the chemical moiety and an agent which upon exposure of the reagent mixture to electrochemical energy forms either a reductant or an oxidant;
- (b) exposing the reagent to electrochemical energy the potential of which oscillates between a potential sufficiently positive to oxidize the chemical moiety and a potential sufficiently negative to reduce the chemical moiety, or to electrochemical energy such that said chemical moiety is oxidized and the agent forms a reductant, or such that said chemical moiety is reduced and the agent forms an oxidant, thereby to induce the chemical moiety to repeatedly electrochemiluminescence;
- and
- (c) detecting emitted luminescence thereby to determine the presence of the chemical moiety.
11 more elements
A method of determining the presence of an analyte of interest which binds to a chemical moiety, which comprises
- (a) forming a reagent mixture comprising the chemical moiety, or comprising the chemical moiety and an agent which upon exposure of the reagent mixture to electrochemical energy forms either a reductant or an oxidant, and the analyte of interest;
- such that the chemical moiety and the analyte specifically bind to one another, said chemical moiety having the formula
- MPL.sup.1 L.sup.2 -(-link-)-!.sub.t B
- wherein M is a lanthanide;
- P is a polydentate ligand of M;
- L.sup.1 and L.sup.2 are ligands of M, each of which may be a substance covalently bound to one or more of P, L.sup.1, or L.sup.2 through one or more covalent bond linkages, said linkages designated as (-link-) and being covalent bonds linking B with at least one of P, L.sup.1 or L.sup.2 ;
- t is an integer equal to or greater than 1;
- B is a biological substance, a synthetic substance which is capable of competing with a biological substance in a competitive binding reaction with a complementary material, said biological substance or synthetic substance being capable of specifically binding to the analyte of interest;
- P, L.sup.1, L.sup.2, and B are of such number that the total number of bonds to M provided by the ligands of M equals the coordination number of M;
- and
- P, L.sup.1, L.sup.2, and B are of such composition that the chemical moiety can be induced to repeatedly emit electromagnetic radiation;
- (b) exposing the reagent mixture to electrochemical energy the potential of which oscillates between a potential sufficiently positive to oxidize the chemical moiety and a potential sufficiently negative to reduce the chemical moiety, or to electrochemical energy such that said chemical moiety is oxidized and the agent forms a reductant, or such that said chemical moiety is reduced and the agent forms an oxidant, thereby to induce the chemical moiety to repeatedly electrochemiluminesce;
- and
- (c) detecting emitted luminescence thereby to determine the presence of the analyte of interest.
11 more elements
A competitive binding method of determining the presence of an analyte of interest wherein the analyte and a chemical moiety bind competitively to a complementary material, which comprises
- (a) forming a reagent mixture comprising the analyte of interest, the complementary material and the chemical moiety, or the chemical moiety and an agent which upon exposure of the reagent mixture to electrochemical energy forms either a reductant or an oxidant, such that the chemical moiety and the analyte of interest bind competitively to the complementary material;
- said chemical moiety having the formula
- MPL.sup.1 L.sup.2 -(-link-)-!.sub.t B
- wherein M is a lanthanide;
- P is a polydentate ligand of M;
- L.sup.1 and L.sup.2 are ligands of M, each of which may be a substance covalently bound to one or more of P, L.sup.1, or L.sup.2 through one or more covalent bond linkages, said linkages designated as (-link-) and being covalent bonds linking B with at least one of P, L.sup.1, or L.sup.2 ;
- t is an integer equal to or greater than 1;
- B is a biological substance or a synthetic substance which is capable of competing with a biological substance in a competitive binding reaction with a complementary material, said biological substance or synthetic substance being capable of binding to the complementary material;
- P, L.sup.1, L.sup.2, and B are of such number that the total number of bonds to M provided by the ligands of M equals the coordination number of M;
- and
- P, L.sup.1, L.sup.2, and B are of such composition that the chemical moiety can be induced to repeatedly emit electromagnetic radiation;
- (b) exposing the reagent mixture to electrochemical energy the potential of which oscillates between a potential sufficiently positive to oxidize the chemical moiety and a potential sufficiently negative to reduce the chemical moiety, or to electrochemical energy such that said chemical moiety is oxidized and the agent forms a reductant, or such that said chemical moiety is reduced and the agent forms an oxidant, thereby to induce the chemical moiety to repeatedly electrochemiluminesce;
- and
- (c) detecting emitted luminescence thereby to determine the presence of the analyte of interest.
11 more elements
A method of determining the presence of a chemical moiety, said chemical moiety having the formula
- MPL.sup.1 L.sup.2 -(-link-)-!.sub.t B
- wherein M is a lanthanide;
- P is a polydentate ligand of M;
- L.sup.1 and L.sup.2 are ligands of M, each of which may be a substance covalently bound to one or more of P, L.sup.1 or L.sup.2 through one or more covalent bond linkages, said linkages designated as (-link-) and being covalent bonds linking B with at least one of P, L.sup.1 or L.sup.2 ;
- t is an integer equal to or greater than 1;
- B is a biological substance, a synthetic substance which is capable of competing with a biological substance in a competitive binding reaction with a complementary material, or a non-biological substance;
- P, L.sup.1 or L.sup.2, and B are of such number that the total number of bonds to M provided by the ligands of M equals the coordination number of m;
- and P, L.sup.1 or L.sup.2, and B are of such composition that the chemical moiety can be induced to repeatedly emit electromagnetic radiation;
- which method comprises:
- (a) forming a reagent mixture containing the chemical moiety;
- (b) exposing the reagent mixture to electrochemical energy the potential of which oscillates between a potential sufficiently positive to oxidize the chemical moiety and a potential sufficiently negative to reduce the chemical moiety, thereby to induce the chemical moiety to repeatedly electrochemiluminesce;
- and
- (c) detecting emitted luminescence thereby to determine the presence of the chemical moiety.
10 more elements
A method of determining the presence of a chemical moiety, said chemical moiety having the formula
- MPL.sup.1 L.sup.2 -(-link-)-!.sub.t B
- wherein M is a lanthanide;
- P is a polydentate ligand of M;
- L.sup.1 and L.sup.2 are ligands of M, each of which may be a substance covalently bound to one or more of P, L.sup.1 or L.sup.2 through one or more covalent bond linkages, said linkages designated as (-link-) and being covalent bonds linking B with at least one of P, L.sup.1 or L.sup.2 ;
- t is an integer equal to or greater than 1;
- B is a biological substance, a synthetic substance which is capable of competing with a biological substance in a competitive binding reaction with a complementary material, or a non-biological substance;
- P, L.sup.1 or L.sup.2, and B are of such number that the total number of bonds to M provided by the ligands of M equals the coordination number of m;
- and P, L.sup.1 or L.sup.2, and B are of such composition that the chemical moiety can be induced to repeatedly emit electromagnetic radiation;
- which method comprises:
- (a) forming a reagent mixture containing the chemical moiety and an agent which upon exposure of the reagent mixture to electrochemical energy forms a reductant;
- (b) exposing the reagent mixture to electrochemical energy such that said chemical moiety is oxidized and the agent forms a reductant, thereby to induce the chemical moiety to repeatedly electrochemiluminesce;
- and
- (c) detecting emitted luminescence thereby to determine the presence of the chemical moiety.
10 more elements
A method of determining the presence of a chemical moiety, said chemical moiety having the formula
- MPL.sup.1 L.sup.2 -(-link-)-!.sub.t B
- wherein M is a lanthanide;
- P is a polydentate ligand of M;
- L.sup.1 and L.sup.2 are ligands of M, each of which may be a substance covalently bound to one or more of P, L.sup.1 or L.sup.2 through one or more covalent bond linkages, said linkages designated as (-link-) and being covalent bonds linking B with at least one of P, L.sup.1 or L.sup.2 ;
- t is an integer equal to or greater than 1;
- B is a biological substance, a synthetic substance which is capable of competing with a biological substance in a competitive binding reaction with a complementary material or a non-biological substance;
- P, L.sup.1 or L.sup.2, and B are of such number that the total number of bonds to M provided by the ligands of M equals the coordination number of m;
- and P, L.sup.1 or L.sup.2, and B are of such composition that the chemical moiety can be induced to repeatedly emit electromagnetic radiation;
- which method comprises:
- (a) forming a reagent mixture containing the chemical moiety and an agent which upon exposure of the reagent mixture to electrochemical energy forms an oxidant;
- (b) exposing the reagent mixture to electrochemical energy such that said chemical moiety is reduced and said agent forms an oxidant, thereby to induce said chemical moiety to repeatedly electrochemiluminescence;
- and
- (c) detecting emitted luminescence thereby to determine the presence of the chemical moiety.
10 more elements
A method of determining the presence of a chemical moiety, said chemical moiety having the formula
- MPL.sup.1 L.sup.2 -(-link-)-!.sub.t B
- wherein M is a lanthanide;
- P is a polydentate ligand of M;
- L.sup.1 and L.sup.2 are ligands of M, each of which may be a substance covalently bound to one or more of P, L.sup.1 or L.sup.2 through one or more covalent bond linkages , said linkages designated as (-link-) and being covalent bonds linking B with at least one of P, L.sup.1 or L.sup.2 ;
- t is an integer equal to or greater than 1;
- B is a biological substance, a synthetic substance which is capable of competing with a biological substance in a competitive binding reaction with a complementary material, or a non-biological substance;
- P, L.sup.1 or L.sup.2, and B are of such number that the total number of bonds to M provided by the ligands of M equals the coordination number of m;
- and P, L.sup.1 or L.sup.2, and B are of such composition that the chemical moiety can be induced to repeatedly emit electromagnetic radiation;
- which method comprises:
- (a) forming an assay reagent mixture containing the chemical moiety and an agent which upon exposure of the reagent mixture to electrochemical energy forms either a reductant or an oxidant;
- (b) exposing the reagent mixture to (i) an electrochemical potential sufficient to reduce said agent to form an oxidant but not sufficient to reduce the chemical moiety to a lower oxidation state, thereby to induce said chemical moiety to electrochemiluminesce or (ii) an electrochemical potential sufficient to oxidize said agent to form a reductant but not sufficient to oxidize said chemical moiety to a higher oxidation state, thereby to induce said chemical moiety to electrochemiluminesce.
8 more elements
A method of determining the presence of an analyte of interest which binds to a chemical moiety, which comprises
- (a) forming a reagent mixture comprising the chemical moiety, or comprising the chemical moiety and an agent which upon exposure of the reagent mixture to electrochemical energy forms either a reductant or an oxidant, and the analyte of interest;
- such that the chemical moiety and the analyte specifically bind to one another, said chemical moiety having the formula
- MPL.sup.1 L.sup.2 -(-link-)-!.sub.t B
- wherein M is a lanthanide;
- P is a polydentate ligand of M ;
- L.sup.1 and L.sup.2 are ligands of M, each of which may be a substance covalently bound to one or more of P, L.sup.1 or L.sup.2 through one or more covalent bond linkages, said linkages designated as (-link-) and being covalent bonds linking B with at least one of P, L.sup.1 or L.sup.2 ;
- t is an integer equal to or greater than 1;
- B is a biological substance, a synthetic substance which is capable of competing with a biological substance in a competitive binding reaction with a complementary material, or a non-biological substance;
- P, L.sup.1 or L.sup.2, and B are of such number that the total number of bonds to M provided by the ligands of M equals the coordination number of m;
- and P, L.sup.1 or L.sup.2, and B are of such composition that the chemical moiety can be induced to repeatedly emit electromagnetic radiation;
- (b) exposing the reagent mixture to (i) an electrochemical potential sufficient to reduce said agent to form an oxidant but not sufficient to reduce the chemical moiety to a lower oxidation state, thereby to induce said chemical moiety to electrochemiluminesce or (ii) an electrochemical potential sufficient to oxidize said agent to form a reductant but not sufficient to oxidize said chemical moiety to a higher oxidation state, thereby to induce said chemical moiety to electrochemiluminesce;
- and
- (c) detecting emitted luminescence thereby to determine the presence of the analyte of interest.
10 more elements
Dependencies are inferred from the claim text.