Methods for inactivating nucleotide sequences and metal chelates for use therein
AssigneeAbbott Laboratories
InventorJoseph E. Celebuski
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Abstract
This invention relates to methods, reagents, and kits utilizing metal chelates to inactivate nucleotide sequences, especially to inactivate polymerase chain reaction (PCR) and ligase chain reaction (LCR) products and to inactivate nucleotide sequences in a bioprocess and bioproduct. A novel metal chelate class is also disclosed.
Claims33
A method for chemically cleaving nucleotide sequences comprising the steps of exposing said nucleotide sequences to an oxidizing agent, a reducing agent, and a metal chelate complex having a formula selected from the group consisting of I, II and III:
- ##STR9## wherein "M" denotes a metal.
A method of chemically cleaving nucleotide sequences, including amplified target nucleotide sequences, comprising the steps of:
- (a) amplifying target nucleotide sequences;
- (b) detecting the amplified target nucleotide sequences;
- and
- (c) chemically cleaving the nucleotide sequences, including the amplified target nucleotide sequences, by exposing them to an oxidizing agent, a reducing agent, and a metal chelate complex of chemical formulae selected from the group consisting of I, II and III:
- ##STR11## wherein "M" denotes a metal.
A metal chelate complex selected from the group consisting of I, II and III with the following formulae:
- ##STR12## wherein "M" denotes a metal.
A kit for chemically cleaving nucleotide sequences comprising:
- a) a container containing a metal chelate complex having chemical formulae selected from the group consisting of I, II, and III:
- ##STR14## wherein "M" denotes a metal;
- and b) another container containing an oxidizing agent.
Dependencies are inferred from the claim text.