US 5,770,157 A · Methods and apparatus for the generation of chemical libraries

Abstract

Methods and apparatus for the generation of chemical libraries are described. The preferred embodiments include a reaction block which uses replaceable reaction chambers supported in the block. Each reaction block is fitted with four sets of 12 reaction chambers, and has fittings that facilitate robotic manipulation. The reaction chambers are preferably fitted with a frit. An s-shaped trap tube snaps into a fitting on the bottom of each reaction chamber. The trap tube runs into a drain tube. The reaction block is fitted with gas (preferably N.sub.2) lines and a septum seal such that gas pressurization empties the reaction chambers into the drain tubes. The drain tubes are arranged so as to mate directly with a standard 96 well microtiter plate for the collection of material. A docking station provides for secure registration of the reaction blocks, and provides for introduction of gases and liquids into the reaction blocks. An inert atmosphere in the reaction block is maintained by a top and (optional) bottom seal. A synthesis support may be introduced into each reaction chamber as a slurry, and the top septum fastened. A needle pipettes reagents from an array of reagent containers into the reaction chambers, and maintains the inert atmosphere. A locking reagent container rack keeps the containers securely in place.

Claims36

Dependent claims

A reaction chamber comprising:

a generally cylindrical upper body having a top opening with a rounded edge, an inner surface, an outer surface, an inside diameter, and a gas inlet port connecting the inner surface of the upper body to the outer surface of the upper body;
a generally cylindrical lower body coaxial with the upper body;
the lower body having an inner surface, an outer surface, and an inside diameter smaller than the inside diameter of the upper body;
a tapered section connecting the upper body to the lower body;
a drain tube having an inside surface and connected to the lower body with a funnel-shaped section;
and
an s-shaped trap tube having an inlet opening and an outlet opening, the inlet opening of the trap tube connected to the drain tube.

4 more elements

Dependencies are inferred from the claim text.

Methods and apparatus for the generation of chemical libraries

Life of this patent

Methods and apparatus for the generation of chemical libraries

Life of this patent

Abstract

Claims36