Method of assay
AssigneeSinvent AS
InventorJomar Frengen
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Abstract
The present invention relates to a binary assay method capable of providing wide dynamic range, a high degree of precision and rapid processing times in which analyte is reacted successively with two independently determinable forms of solid-supported binding partner and also with a labelled ligand having affinity for the analyte or binding partner. The analyte concentration is determined from signals deriving from the resulting two forms of analyte-binding partner-labelled ligand systems by reference to a double standard calibration curve. A kit for use in the method is also disclosed.
Claims12
In a method for assaying an analyte in a sample comprising reacting the sample with two independently determinable forms of solid-supported binding partner having affinity for the analyte and with a labelled ligand having affinity for the analyte or the binding partner, thereby yielding two forms of labelled ligand-carrying solid-supported binding partner, said two independently determinable forms of solid-supported binding partner being such that signals in respect of said two forms of labelled ligand-carrying solid-supported binding partner may be independently determined whereby the analyte concentration may be obtained by reference to a double standard calibration curve, wherein the improvement comprises adding to the sample the first form of solid-supported binding partner and then after an interval adding the second form of solid-supported binding partner.
Dependencies are inferred from the claim text.