Ocs-element
AssigneesAgrigenetics, Inc., Commonwealth Scientific and Industrial Research Organization
InventorsJeff G. Ellis, Daniel J. Llewellyn, W. James Peacock, Elizabeth Dennis, David Bouchez
Life of this patent
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Abstract
A DNA fragment is provided which is a plant enhancer element capable of activating or enhancing the transcription level of a plant-expressible gene consisting essentially of a consensus sequence selected from the group consisting of ##STR1## and its reverse sequence. Said DNA fragment may also contain a second sequence 5'-ACGTAAGCGCTTACGT-3'. These sequences bind with ocs transcription factor.
Claims34
A recombinant DNA molecule comprising a DNA fragment which fragment is a plant enhancer element capable of being bound by an OCS transcription factor and displaying the upper band binding pattern characteristic of the wild-type ocs enhancer in gel retardation assays, said fragment comprising a consensus sequence selected from the group consisting of ##STR4## and its reverse sequence, in combination with (a) a plant-expressible promoter heterologous to said plant enhancer element wherein said promoter is placed 3' to said enhancer element, and
- (b) a plant-expressible structural gene wherein said gene is placed 3' to said promoter and under the regulatory control of said enhancer element and said plant-expressible promoter.
A recombinant DNA molecule comprising a DNA fragment which fragment is a plant enhancer element capable of being bound by an OCS transcription factor and displaying the upper band binding pattern characteristic of the wild-type ocs enhancer in gel retardation assays, wherein said fragment comprises the sequence 5'-ACGTAAGCGCTFACGT-3', and its reverse sequence, in combination with
- (a) a plant-expressible promoter heterologous to said plant enhancer element wherein said promoters placed 3' to said enhancer element, and
- (b) a plant-expressible structural gene wherein said gene is placed 3' to said promoter and under the regulatory control of said enhancer element and said plant-expressible promoter.
A recombinant DNA molecule comprising a DNA fragment which fragment is a plant enhancer element capable of being bound by an OCS transcription factor and displaying the upper band binding pattern characteristic of the wild-type ocs enhancer in gel retardation assays, said fragment comprising at least two consensus sequences selected from the group consisting of ##STR5## and its reverse sequence, in combination with (a) a plant-expressible promoter placed 3' to said enhancer element, and
- (b) a plant-expressible structural gene wherein said gene is placed 3' to said promoter and under the regulatory control of said enhancer element and said plant-expressible promoter.
A recombinant DNA molecule comprising a DNA fragment which fragment is a plant enhancer element capable of being bound by an OCS transcription factor and displaying the upper band binding pattern characteristic of the wild-type enhancer in gel retardation assays, wherein said fragment comprises the sequence 5'-ACGTAAGCGCTACGT-3', and its reverse sequence, in combination with
- (a) a plant-expressible promoter heterologous to said plant enhancer element wherein said promoter is placed 3' to said enhancer element, and
- (b) a plant-expressible structural gene wherein said gene is placed 3' to said promoter and under the regulatory control of said enhancer element and said plant-expressible promoter.
Dependencies are inferred from the claim text.