Gene encoding melanocortin-5 receptor and methods of use
AssigneeThe Regents of the University of Michigan
InventorsTadataka Yamada, Ira Gantz
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Abstract
Genes encoding melanocortin receptors have been identified, isolated, cloned and localized to their chromosomal positions. These genes have been used to transfect mammalian cells lacking endogenous melanocortin receptors to induce expression. Additionally, melanocortin receptor binding, secondary signalling, and tissue distribution has been characterized. The genes and their gene products may therefore be used to to provide therapeutic vehicles for the treatment of processes involving the function of melanocortin receptors.
Claims16
An isolated nucleic acid molecule encoding the amino acid sequence of Sequence Listing ID No. 10.
An isolated nucleic acid molecule comprising a nucleotide sequence which hybridizes to the nucleic acid comprising the nucleotide sequence of Sequence Listing ID No. 9 in 6.times.SSC at 42.degree. C. followed by washing with 1.times.SSC at 55.degree. C. wherein said isolated nucleic acid molecule encodes a melanocortin-5 receptor.
An isolated nucleic acid molecule comprising a nucleotide sequence which hybrizes to the nucleic acid comprising the complementary nucleotide sequence of Sequence Listing ID No. 9 in 6.times.SSC at 42.degree. C. followed by washing with 1.times.SSC at 55.degree. C., wherein said isolated nucleic acid molecule encodes a melanocortin-5 receptor.
A method for assaying the binding of a ligand to a melanocortin-5 receptor comprising the steps of:
- a) transfecting a cell which does not endogenously express melanocortin-5 receptor with a vector comprising an isolated nucleic acid molecule comprising a nucleotide sequence which hybridizes to the nucleic acid comprising the nucleotide sequence of Sequence Listing ID No. 9 in 6.times.SSC at 42.degree. C. followed by washing with 1.times.SSC at 55.degree. C., wherein said isolated nucleic acid molecule encodes a melanocortin-5 receptor, under conditions favorable for expression of melanocortin-5 receptor;
- b) contacting the cell with a ligand;
- and
- c) detecting ligand binding to the expressed melanocortin-5 receptor.
Dependencies are inferred from the claim text.