Purification of von Willebrand factor by affinity chromatography
AssigneeRhone-Poulenc Rorer Pharmaceuticals Inc.
InventorsJack Newman, David Farb
Life of this patent
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Abstract
A method of improving the therapeutic activity of von Willebrand Factor obtained from materials comprising on Willebrand Factor comprising incubating said Factor at a temperature of about 20.degree. C. to about 55.degree. C. for about 1 to about 30 hours, most preferably at from about 45.degree. C. to about 55.degree. C. for about 1 to about 5 hours.
Claims6
A method of improving the therapeutic activity of von Willebrand Factor prepared by recombinant technology, the improvement comprising:
- incubating a solution of said Factor, substantially free from a chaotropic agent and Factor VIII and not in contact with a column matrix solid substance, at a temperature of about 20.degree. C. to about 55.degree. C. for about 1 to about 30 hours at a pH of about 6.5 to 7.5 and with buffering, which conditions increase the activity of said Factor.
A method of improving the therapeutic activity of von Willebrand Factor prepared by recombinant technology and eluted from a material comprising said Factor bound to a monoclonal or polyclonal antibody specific thereto, the improvement comprising:
- incubating a solution of said Factor, substantially free from a chaotropic agent and Factor VIII and not in contact with a column matrix solid substance, at a temperature of about 20.degree. C. to about 55.degree. C. for about 1 to about 30 hours at a pH of about 6.5 to 7.5 and with buffering, which conditions increase the activity of said Factor.
Dependencies are inferred from the claim text.