US 5,710,251 A · Purification of bacterially expressed human interleukin-10

Abstract

Disclosed is a method for purifying Interleukin-10 (IL-10). The method is comprised of subjecting an IL-10 containing solution to cation exchange chromatography, anion exchange chromatography, hydroxyapatite chromatography, and gel filtration chromatography. The present invention is also comprised of a process for separating different IL-10 dimers present in a protein fraction from each other by subjecting the protein fraction to hydroxyapatite chromatography. The present invention is also comprised of a process for separating variants of a protein differing in an N-terminal amino acid sequence present in a protein fraction from each other by subjecting the protein fraction to hydroxyapatite chromatography.

Claims9

Dependent claims

A method for purifying bacterially expressed human Interleukin-10 (IL-10) contained within a solution comprising:

(a) applying a solution containing IL-10 to a cation exchange chromatography column thereby obtaining fractions containing IL-10;
(b) applying the IL-10-containing fractions from step (a) to an anion exchange chromatography column thereby obtaining fractions containing IL-10;
(c) applying the IL-10-containing fractions from step (b) to a hydroxyapatite chromatography chromatography thereby obtaining fractions containing a single isolated dimer of IL-10.

Dependencies are inferred from the claim text.

Purification of bacterially expressed human interleukin-10

Life of this patent

Purification of bacterially expressed human interleukin-10

Life of this patent

Abstract

Claims9