HSP-60 genomic locus and primers for species identification
AssigneesNational Research Council, The University of British Columbia
InventorsSwee Han Goh, Anthony W. Chow, Sean Hemmingsen
Life of this patent
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Abstract
The present invention provides oligonucleotide primers and a method of using these primers for identification of the species of an organism, wherein the identification includes amplification of a variable polynucleotide sequence encoding a highly conserved region of a heat shock polypeptide.
Claims10
A genomic polynucleotide locus which is defined by being amplified by primers having a sequence:
- 5'-GAIIIIGCIGGIGA(T/C)GGIACIACIAC-3' (SEQ. ID NO:
- 3) and
- 5'-(T/C)(T/G)I(T/C)(T/G)ITCICC(A/G)AAICCIGGIGC(T/C)TT-3' (SEQ ID NO:4)
- wherein the amplified polynucleotide locus from one species does not hybridize under high stringency conditions with a polynucleotide locus from another species amplified by primers having a sequence:
- 5'-GAIIIIGCIGGIGA(T/C)GGIACIACIAC-3' (SEQ ID NO:3) and
- 5'-(T/C)(T/G)I(T/C)(T/G)ITCICC(A/G)AAICCIGGIGC(T/C)TT-3' (SEQ ID NO:4).
- 2.
A kit useful for the isolation of target DNA for identification of the species of an organism, the kit comprising means for amplifying target DNA, said means comprising the necessary enzyme(s) and oligonucleotide primers for amplifying said target DNA from the organism or a cell of the organisms said primers consisting of a sequence:
- 5'-GAIIIIGCIGGIGA(T/C)GGIACIACIAC-3' (SEQ ID NO:3) and
- 5'-(T/C)(T/G)I(T/C)(T/G)ITCCC(A/G)AAICCIGGIGC(T/C)TT-3' (SEQ ID NO:4).
- 10.
Dependencies are inferred from the claim text.