Probe for diagnosing infectious diseases which hybridizes with DNA from candida albicans
AssigneesTsuneya Ohno, Fuso Pharmaceutical Industries, Ltd.
InventorsTsuneya Ohno, Takuo Hirotsu, Hiroyuki Keshi, Akio Matsuhisa
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Abstract
The invention provides a probe useful for rapidly detecting and identifying fungi causative of infectious diseases by digesting completely a DNA extracted from a fungus, e.g., Candida albicans, with EcoRI, inserting the same into an appropriate cloning vector, and selecting a probe containing DNA fragments unique to each fungus. The invention also provides a standard sequence suitable for making reference to a genomic DNA contained in a clinical specimen by elucidating the base sequence of the probe thus selected.
Claims13
A purified and isolated C. albicans nucleic acid fragment useful for diagnosing infectious diseases, said nucleic acid comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 3, and SEQ ID NO:
- 4.
A purified and isolated polynucleotide probe for diagnosing infectious diseases, said probe comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 3 and SEQ ID NO:
- 4, said probe being capable of hybridizing with DNA from Candida albicans and failing to hybridize to human genomic DNA under the following hybridization conditions:
- (a) prehybridization/hybridization buffer comprising 45% formamide and 5.times.SSC;
- (b) prehybridization at 42.degree. C. for two hours;
- (c) hybridization overnight at 42.degree. C.;
- (d) washing for 10 minutes at 50.degree. C. using a wash solution comprising 1.times.SSC and 0.1% SDS;
- (e) washing for 10 minutes at 50.degree. C. using a wash solution comprising 0.5.times.SSC and 0.1% SDS.
A purified and isolated nucleic acid useful for diagnosing infectious diseases, said nucleic acid consisting of a nucleotide sequence derived from C. albicans and at least one heterologous nucleotide sequence, said nucleotide sequence derived from C. albicans consisting of one of SEQ ID NO:
- 1, SEQ ID NO:
- 3, and SEQ ID NO:
- 4.
Dependencies are inferred from the claim text.