Epidermal growth factor receptor-like gene product and its uses
OwnerJohn V. Ilekisinventor
Life of this patent
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Abstract
A novel human epidermal growth factor receptor-like protein has been identified, designated TEGFR. Provided herein is the nucleic acid sequence and deduced amino acid sequence of this protein. The invention relates to the measurement of its protein by immunological means or the measurement of its mRNA by recombinant DNA means in human biological samples. The invention also pertains to the use of its cDNA to produce a recombinant source of the protein. Moreover, the invention relates to its therapeutic use to inhibit the activity of epidermal growth factor receptor. Lastly, the invention also concerns the diagnostic and therapeutic use of this protein for human cancers.
Claims16
An isolated nucleic acid selected from the group consisting of:
- nucleotides 1 through 105 and nucleotides 1713 through 1828 of SEQ ID NO:1.
An isolated nucleic acid selected from the group consisting of the complement of:
- nucleotides 1 through 105 of SEQ ID NO:1 or fragments of at least 16 nucleotides in length thereof and nucleotides 1713 through 1828 of SEQ ID NO:1 or fragments of at least 16 nucleotides in length thereof.
An isolated polypeptide encoded by SEQ ID NO:1.
A method for the production of polyclonal or monoclonal antibodies directed against the unique carboxy terminus, SEQ ID NO:2, of the polypeptide encoded by the isolated DNA of SEQ ID NO:1 using as an antigen a peptide consisting of at least 6 amino acids and comprising the amino acid sequence of SEQ ID NO:2 used alone or conjugated to a suitable carrier as a means for eliciting an immune response, comprising the following steps:
- a) immunizing a host animal with said antigen in suitable vehicle,
- b) harvesting antibodies from said host's blood or harvesting antibodies produced by said host's antibody-producing cells or said host's antibody-producing cells fused with another cell as a means of producing antibodies outside the original said host, said antibodies directed against the unique carboxy terminus of the polypeptide encoded by the isolated DNA of SEQ ID NO:1.
A method for detecting the nucleic acid of SEQ ID NO:1, referred to as target nucleic acid, in a human biological specimen using a nucleic acid isolate selected from the group consisting of nucleotides 1 through 105 of SEQ ID NO:1 or fragments of 16 nucleotides in length thereof and nucleotides 1713 through 1828 of SEQ ID NO:1 or fragments of 16 nucleotides in length thereof, comprising the following steps:
- a) optionally separating the said target nucleic acid in said specimen from interfering related nucleic acids by a means based on size, charge or weight,
- b) adding said nucleic acid isolate to said biological specimen containing the nucleic acid encoded by SEQ ID NO:1 in the form of RNA or DNA,
- c) allowing said nucleic acid isolate to hybridize or anneal to said target nucleic acid,
- d) measuring the amount of hybridized or annealed nucleic acid complex or measuring the amount of a nucleic acid product formed that is derived from the target nucleic acid, initially present in said specimen, following amplifying said nucleic acid product.
Dependencies are inferred from the claim text.