US 5,707,875 A · 170-Labeled phosphoric acid compound and method and apparatus for selective observation of nuclear magnetic resonance signals using the compound

Abstract

Using a mixed sample of a plurality of phosphoric acid compounds different in chemical structure as a sample for measurement, a .sup.31 P nuclear magnetic resonance signal obtained through polarization transfer between .sup.17 O and .sup.31 P is observed and signals obtained without polarization transfer are eliminated, whereby .sup.31 P nuclear magnetic resonance signal spectrum of an .sup.17 O-labeled phosphoric acid is selectively observed. For this purpose, a double resonance NMR detector capable of exciting .sup.17 O and .sup.31 P at the same time is connected to an NMR spectrometer, and using a pulse train which permits observation of a .sup.31 P nuclear magnetic resonance signal obtained through polarization transfer between .sup.17 O and .sup.31 P, a .sup.31 P nuclear magnetic resonance signal from the .sup.17 O-labeled phosphoric acid is observed, and signals for .sup.17 O-unlabeled .sup.31 P are eliminated.

Claims15

Dependent claims

A method for measuring nuclear magnetic resonance comprising

labeling a phosphoric acid group contained in a sample with at least .sup.17 O , one atom and
detecting a nuclear magnetic resonance signal due to the labeled phosphoric acid group in distinction from nuclear magnetic resonance signals due to unlabeled phosphoric acid groups by utilizing polarization transfer attributable to a spin-spin coupling between .sup.31 P and .sup.17 O.

A method for selective observation of a nuclear magnetic resonance signal which comprises

using as a sample for measurement a material containing an .sup.17 O-labeled phosphoric acid compound containing at least one .sup.17 O atom in the phosphoric acid group at a rate higher than the natural isotopic abundance of .sup.17 O and a phosphoric acid compound containing .sup.17 O present at the natural isotopic abundance,
eliminating .sup.31 P nuclear magnetic resonance signals made by the .sup.17 O-unlabeled phosphoric acid compound by using a pulse sequence which permits observation of a .sup.31 P nuclear magnetic resonance signal obtained through polarization transfer attributable to spin-spin coupling between .sup.17 O and .sup.31 P, and
observing a .sup.31 P nuclear magnetic resonance signal made by the .sup.17 O-labeled phosphoric acid compound.

An apparatus for selective observation of a .sup.31 P nuclear magnetic resonance signal which comprises

a container to accommodate a sample for measurement containing an .sup.17 O-labeled phosphoric acid compound having at least one .sup.17 O atom in its phosphoric acid group at a rate higher than the natural isotopic abundance of .sup.17 O and a phosphoric acid compound containing .sup.17 O present at the natural isotopic abundance,
an irradiating coil for exciting .sup.17 O magnetically by a predetermined pulse sequence,
an irradiating coil and detecting coil for exciting and observing .sup.31 P magnetically by a predetermined pulse sequence,
at least two transmitters for applying voltages of predetermined frequencies to each of the irradiating coils for .sup.17 O and .sup.31 P,
a receiver in which nuclear magnetic resonance signals from the sample through the observing coil are detected, and the detected nuclear magnetic resonance signals are subjected to a predetermined processing, followed by observing a .sup.31 P nuclear magnetic resonance signal from the .sup.17 O-labeled phosphoric acid compound, and
a means for applying a predetermined magnetic field to the sample and the coils.

3 more elements

Dependencies are inferred from the claim text.

170-Labeled phosphoric acid compound and method and apparatus for selective observation of nuclear magnetic resonance signals using the compound

Life of this patent

170-Labeled phosphoric acid compound and method and apparatus for selective observation of nuclear magnetic resonance signals using the compound

Life of this patent

Abstract

Claims15