US 5,707,851 A · Gram-positive alkaliphilic microorganisms

Abstract

The present invention provides novel aerobic, Gram-positive alkaliphilic bacteria which have been isolated from in and around alkaline soda lakes. These alkaliphiles have been analyzed according to the principles of numerical taxonomy with respect to each other and also to a collection of known bacteria. In addition, these bacterial taxa are further circumscribed by an analysis of the lipid components which serve as chemotaxonomic markers. The alkaliphiles of the present invention produce alkalitolerant enzymes which are capable of performing their functions at high pH which makes them uniquely suited for applications requiring such extreme conditions.

Claims4

Dependent claims

Cells of a single strain of bacteria obtained from a culture consisting of said single strain of bacteria, which are useful for the production of alkalitolerant enzymes, wherein the bacteria consist of aerobic, Gram-positive, short, irregular, rod-shaped, obligate alkaliphilic bacteria having the following characteristics:

a) forms cream-yellow, small, circular colonies;
b) grows optimally above pH 8;
c) gives a positive response to the following tests:
1) Gelatin hydrolysis
2) Galactose
3) .beta.-glucosidase
4) Arginine
5) Bacitracin
6) N-acetyl-D-glucosamine
7) Cellobiose
8) Inosine
9) Uridine;
and
d) gives a negative response to the following tests:
1) Maltose
2) Acetate
3) D-glucose
4) D-melibiose
5) Propionate
6) Valerate
7) Glycogen
8) Serine
9) Ampicillin
10) Penicillin G
11) Methicillin
12) Tetracyclin
13) Methyl pyruvate
14) Mono-methylsuccinate
15) .alpha.-ketobutyric acid.

26 more elements

Dependencies are inferred from the claim text.

Gram-positive alkaliphilic microorganisms

Life of this patent

Gram-positive alkaliphilic microorganisms

Life of this patent

Abstract

Claims4