Multifunctional RNA having self-processing activity, the preparation thereof and the use thereof
AssigneeHoechst Aktiengesellschaft
InventorsHubert Mullner, Eugen Uhlmann, Peter Eckes, Rudolf Schneider, Bernadus Uijtewaal
Life of this patent
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Abstract
A multifunctional RNA having self-processing activity, the preparation thereof and the use thereof. Host cells can be transformed so that they express ribozyme RNA and antisense RNA which are connected with each other via a spacer. The RNA molecules can, for example, be complementary to a certain viral RNA. Plants which have been transformed with genes coding for RNA of this type show a significantly improved resistance to viruses.
Claims10
An isolated DNA fragment which encodes a pattern which comprises:
- 5'-hammerhead ribozyme RNA-(spacer-antisense RNA).sub.n -spacer-hammerhead ribozyme RNA-3', wherein n is a number from 0 to 10, and the spacer is a chain of 20 to 25 nucleotides which contains a ribozyme cleavage site in each case;
- and wherein said DNA fragment encodes a multifunctional RNA with self-processing activity.
The isolated DNA fragment which comprises the DNA SEQUENCE ID NO. 1.
Dependencies are inferred from the claim text.