Diagnosis of Mycobacterium bovis infection
AssigneeCommonwealth Scientific and Industrial Research Organisation
InventorsPaul Richard Wood, Anthony John Radford, Theodora Fifis
Life of this patent
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Abstract
A method for the diagnosis of Mycobacterium bovis infection in a susceptible animal, comprises detection in said animal of antibodies against the MPB-70 protein of M.bovis and/or the detection of a cell-mediated immune response of said animal to the said MPB-70 protein. Also disclosed is a recombinant DNA molecule corresponding to all or portion of the M.bovis DNA sequence coding for the MPB-70 protein or a polypeptide having the antigenicity of MPB-70 protein, or degenerate forms thereof, as well as recombinant MPB-70 protein or polypeptide and a process for the preparation thereof.
Claims11
A method for the detection of Mycobacterium bovis infection in a susceptible animal, which comprises detecting in said animal an immune response to MPB-70 protein of M.bovis having the amino acid sequence of FIG. 3 by means of an assay selected from the group consisting of an assay for MPB-70 recognizing antibodies and an assay for a cell-mediated immune response;
- said assay using said MPB-70 protein or an antigenic polypeptide derived therefrom reactive with affinity-purified antibody to M. bovis as antigen, and said MPB-70 protein or antigenic polypeptide derived therefrom being substantially free of other M.bovis proteins.
A kit for the detection of Mycobacterium bovis infection in a susceptible animal, which comprises means for the detection in said animals of an immune response to MPB-70 protein of M.bovis having the amino acid sequence of FIG. 3 by means of an assay selected from the group consisting of an assay for MPB-70 recognizing antibodies and an assay for cell mediated immune response, said kit comprising said MPB-70 protein or an antigenic polypeptide derived therefrom reactive with affinity-purified antibody to M.bovis as antigen, and said MPB-70 protein or antigenic polypeptide derived therefrom being substantially free of other M.bovis proteins.
A method for the detection of Mycobacterium bovis infection in a susceptible animal which comprises detecting in said animal an immune response to MPB-70 protein of M.bovis having the amino acid sequence of FIG. 3;
- said immune response being detected by contacting a serum sample from said animal with an antigen selected from the group consisting of said MPB-70 protein and antigenic polypeptides derived therefrom reactive with affinity-purified antibody to M.bovis;
- and detecting binding of antibodies in said sample with said antigen to indicate the presence of said antibodies in said sample;
- said MPB-70 protein or antigenic polypeptide derived therefrom being substantially free of other M.bovis proteins.
A method for the detection of Mycobacterium bovis infection in a susceptible animal which comprises detecting in said animal an immune response to MPB-70 protein of M.bovis having the amino acid sequence of FIG. 3;
- said immune response being detected by contacting a serum sample from said animal with an antigen selected from the group consisting of said MPB-70 protein and antigenic polypeptides derived therefrom reactive with affinity-purified antibody to M.bovis;
- and detecting binding of antibodies in said sample with said antigen by competition with binding of labelled antibody to said MPB-70 protein to indicate the presence of said antibodies in said sample;
- said MPB-70 protein or antigenic polypeptide derived therefrom being substantially free of other M.bovis proteins.
MPB-70 protein of M.bovis having the amino acid sequence of FIG. 3, or an antigenic polypeptide derived therefrom reactive with affinity-purified antibody to M.bovis, said MPB-70 protein or antigenic polypeptide derived therefrom being substantially free of other M.bovis proteins.
Dependencies are inferred from the claim text.