Carbohydrate-based vaccine and diagnostic reagent for trichinosis
AssigneeColorado State University Research Foundation
InventorsNancy Wisnewski, Robert B. Grieve, Donald L. Wassom, Michael R. McNeil
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Abstract
The present invention relates to Trichinella diagnostic reagents that include a .beta.-tyvelose-containing composition and use of such reagents to detect Trichinella, and particularly Trichinella spiralis infections. The present invention also includes diagnostic kits based on such reagents and therapeutic agents based on the the knowledge that .beta.-tyvelose is produced by Trichinella spiralis parasites.
Claims25
A diagnostic reagent to detect Trichinella infection comprising .beta.-tyvelose joined through glycosidic linkage to at least one monosaccharide to form an oligosaccharide having at least one .beta.-tyvelose terminal residue.
A method to detect Trichinella infection in an animal comprising:
- (a) contacting a bodily fluid collected from said animal with a diagnostic reagent comprising a .beta.-tyvelose-containing composition to form an immunocomplex between said diagnostic reagent and an antibody in said bodily fluid indicative of Trichinella infection, said .beta.-tyvelose-containing composition comprising .beta.-tyvelose joined through glycosidic linkage to at least one monosaccharide to form an oligosaccharide having at least one .beta.-tyvelose terminal residue;
- and
- (b) determining Trichinella infection by detecting the presence of said immunocomplex formed in step (a), wherein the presence of said immunocomplex indicates Trichinella infection.
A diagnostic kit for detecting Trichinella infection in an animal, said kit comprising (a) a diagnostic reagent comprising .beta.-tyvelose joined through glycosidic linkage to at least one monosaccharide to form an oligosaccharide having at least one .beta.-tyvelose terminal residue:
- and (b) a means for detecting Trichinella infection.
Dependencies are inferred from the claim text.