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US 5,707,812 AGranted
Purification of plasmid DNA during column chromatography
AssigneeVical Incorporated
InventorsNancy Horn, Greg Budahazi, Magda Marquet
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Abstract
A method for purifying plasmid DNA during column chromatography is provided. A short chain polymeric alcohol, preferably polyethylene glycol, or another DNA condensation agent, is added to a DNA sample prior to column chromatography. The short chain polymeric alcohol or condensation agent promotes improved isolation of plasmid DNA and may be used for large scale purification, particularly for manufacturing plasmid DNA as a biopharmaceutical.
Claims11
A method of purifying plasmid DNA from lysate contaminants comprising:
- (a) preparing a lysate;
- (b) clarifying said lysate to produce a clarified lysate;
- (c) adding a short chain polymeric alcohol to said clarified lysate;
- (d) binding plasmid DNA present in said clarified lysate to a chromatographic matrix by placing said lysate in contact with said chromatographic matrix;
- and
- (e) eluting said plasmid DNA by placing an eluent in contact with said chromatographic matrix.
3 more elements
A method of purifying plasmid DNA from lysate contaminants comprising:
- (a) preparing a lysate;
- (b) clarifying said lysate to produce a clarified lysate;
- (c) adding a short chain polymeric alcohol to said clarified lysate;
- (d) binding plasmid DNA present in said clarified lysate to an ion exchange chromatographic matrix by placing said lysate in contact with said chromatographic matrix;
- and
- (e) eluting said plasmid DNA by placing an eluent in contact with said chromatographic matrix.
3 more elements
Dependencies are inferred from the claim text.