Bacillus thuringiensis isolates active against weevils
AssigneeMycogen Corporation
InventorsGregory A. Bradfisch, H. Ernest Schnepf, Leo Kim
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Abstract
The subject invention concerns the discovery of Bacillus thuringiensis isolates with advantageous activity against weevils. In preferred embodiments of the invention, B.t. isolates, or toxins therefrom, are used to control alfalfa weevils, boll weevils, and/or rice water weevils. The toxins can be administered to the pests through a variety of methods including the transformation of bacteria or plants to produce the weevil-active toxins.
Claims17
A method of killing rice water weevils which comprises administering to rice water weevils a pesticidally effective amount of a microbial isolate that expresses a Bacillus thuringiensis (B.t.) .delta.-endotoxin, wherein said isolate is selected from the group consisting of MR506, KB92, PS201T6, PS101Z2, PS50B, PS33F2, PS204G4, PS204G6, PS167P, PS192N1, PS201L1, PS169E, PS177G, PS196S1, and PS73E;
- or a .delta.-endotoxin therefrom;
- or a weevil killing fragment of said .delta.-endotoxin.
A method of killing rice water weevils which comprises administering to rice water weevils a pesticidally effective amount of a Bacillus thuringiensis (B.t.) isolate or a .delta.-endotoxin therefrom wherein the Bacillus thuringiensis .delta.-endotoxin is a cyt toxin, or a weevil killing fragment thereof.
A method of killing weevils which comprises administering to said weevils a pesticidally effective amount of a Bacillus thurigiensis isolate, wherein said isolate is selected from the group consisting of PS201T6, KB92, PS50B, PS204G4, PS204G6, PS167P, PS192N1, PS201L1, PS169E, PS177G, PS196S1, and PS73E;
- or a .delta.-endotoxin therefrom;
- or a weevil killing fragment of said .delta.-endotoxin.
A method of killing boll weevils (Anthonomus grandis) which comprises contacting said weevils with a pesticidally effective mount of a microbial isolate that expresses a Bacillus thuringiensis .delta.-endotoxin, wherein said isolate is selected from the group consisting of PS33F2, MR506, and PS101Z2;
- or a .delta.-endotoxin therefrom;
- or a weevil killing fragment of said .delta.-endotoxin.
A composition of matter comprising a Bacillus thuringiensis (B.t. ) isolate or spores, crystals, or .delta.-endotoxins from a B.t. isolate, or a recombinant microbe that expresses a B.t. toxin, in association with a formulation particularly adapted for use with rice water weevils, wherein said B.t. isolate or said recombinant microbe is selected from the group consisting of MR506, KB92, PS201T6, PS101Z2, PS50B, PS33F2, PS204G4,PS204G6, PS167P, PS192N1, PS201L1, PS169E, PS177G, PS196S1, and PS73E.
Dependencies are inferred from the claim text.