Channel plate for DNA sequencing
AssigneeBattelle Memorial Institute
InventorsRichard J. Douthart, Shannon L. Crowell
Life of this patent
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Abstract
This invention is a channel plate that facilitates data compaction in DNA sequencing. The channel plate has a length, a width and a thickness, and further has a plurality of channels that are parallel. Each channel has a depth partially through the thickness of the channel plate. Additionally an interface edge permits electrical communication across an interface through a buffer to a deposition membrane surface.
Claims18
A channel plate for electrophoresis sequencing of a DNA sample, said channel plate having (a) a length, a width and a thickness, and further having, (b) a plurality of channels that are parallel placed upon an upper surface of the channel plate, each channel having a depth partially through the thickness and filled with a gel, wherein the improvement comprises:
- (c) said channel plate is made from electrically non-conductive, non-cellulosic material, and
- (d) a temperature control plate attached to a lower surface wherein a temperature of the channel plate is controlled.
A method of preparing a DNA sample for electrophoretic separation, comprising the steps of:
- (a) providing a glass ceramic channel plate having a length, width and thickness, further having a plurality of parallel channels formed partially through the thickness and each channel having a first end at a position distant from a first edge, each of the channels extending therefrom to an interface edge;
- (b) filling the channels with a gel;
- (c) placing a cover plate over the channels thereby covering the channels and confining the gel within the channels;
- (d) inserting a comb into the channels;
- (e) permitting the gel to polymerize with the comb in place;
- (f) withdrawing the comb after polymerization of the gel and leaving, sample wells;
- and
- (g) filling the sample wells with DNA sample.
A channel plate for electrophoresis sequencing of a DNA sample, said channel plate having (a) a length, a width and a thickness, and further having, (b) a plurality of channels that are parallel placed upon an upper surface of the channel plate, each channel having a depth partially through the thickness and filled with a gel, wherein the improvement comprises:
- (c) said channel plate is made from a glass ceramic.
A method of preparing a DNA sample for electrophoretic separation, comprising the steps of:
- (a) providing an electrically non-conducting, non-cellulosic channel plate having a length, width and thickness, further having a plurality of parallel channels formed partially through the thickness and each channel having a first end at a position distant from a first edge, each of the channels extending therefrom to an interface edge;
- (b) filling the channels with a gel;
- (c) placing a cover plate over the channels thereby covering the channels and confining the gel within the channels;
- (d) inserting a comb into the channels;
- (e) permitting the gel to polymerize with the comb in place;
- (f) withdrawing the comb after polymerization of the gel and leaving the sample wells;
- (g) filling the sample wells with DNA sample;
- and
- (h) attaching a temperature control plate to a lower surface of said channel plate for controlling a temperature of said channel plate during the electrophoretic separation.
Dependencies are inferred from the claim text.