Method of directly freezing porcine embryos
AssigneeThe Japanese Research Association for Animal Embryo Transfer Technology
InventorKenji Kameyama
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Abstract
A method of directly freezing porcine embryos which includes adding a solution to be frozen including ethylene glycol, propylene glycol, bovine serum and dextran in the presence of basic medium to porcine embryos and freezing the resulting mixture.
Claims9
A method of freezing a porcine embryo which comprises freezing a porcine embryo in a solution comprising ethylene glycol, propylene glycol, bovine serum, dextran and a basic medium.
A method of propagating a pig which comprises freezing a porcine embryo in a cryoprotectant solution comprising bovine serum, ethylene glycol, propylene glycol, and dextran, thereby forming a frozen porcine embryo, thawing the frozen porcine embryo and transferring the thawed porcine embryo to a recipient animal.
A method of freezing a porcine embryo which comprises freezing a porcine embryo in a cryoprotectant solution comprising bovine serum, ethylene glycol, propylene glycol, and dextran.
Dependencies are inferred from the claim text.