US 5,705,627 A · Detection of human papillomavirus by the polymerase chain reaction using specific L1, and E6 probes

Abstract

The presence of human papillomavirus (HPV) in a sample can be detected and the HPV typed by a method that involves the amplification of HPV DNA sequences by the polymerase chain reaction (PCR). The primers used in the method are consensus primers that can be used to amplify a particular region of the genome of any HPV. The presence of HPV in a sample is indicated by the formation of amplified DNA. The HPV nucleic acid is detected by consensus probes that may be short oligonucleotide probes or long generic probes. The HPV is typed by the use of type-specific DNA probes specific for the amplified region of DNA.

Claims8

Dependent claims

An HPV L1 type-specific probe selected from the group consisting of:

MY12, MY13, MY14, MY16, MY58, MY59, MY60, MY61, MY62, MY63, MY64, MY65, MY71 (Seq ID No. 12), MY72 (Seq ID No. 13), MY73 (Seq ID No. 18), MY83 (Seq ID No. 68), MY84 (Seq ID No. 84), MY85 (Seq ID No. 71), MY86 (Seq ID No. 70), MY95 (Seq ID No. 14), MY96 (Seq ID No. 15), MY97 (Seq ID No. 16), MY104 (Seq ID No. 74), MY105 (Seq ID No. 75), MY106 (Seq ID No. 76), MY107 (Seq ID No. 19), MY125 (Seq ID No. 10), MY126 (Seq ID No. 11), MY130 (Seq ID No. 20), MY131 (Seq ID No. 21), MY132 (Seq ID No. 22), MY133 (Seq ID No. 17), WD74, WD75 (Seq ID No. 23), WD150, WD151, and sequences fully complementary thereto.

Dependencies are inferred from the claim text.

Detection of human papillomavirus by the polymerase chain reaction using specific L1, and E6 probes

Life of this patent

Detection of human papillomavirus by the polymerase chain reaction using specific L1, and E6 probes

Life of this patent

Abstract

Claims8