Biologically active polypeptide fusion dimers
AssigneeAmgen Inc.
InventorArlen R. Thomason
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Abstract
The present invention provides a biologically active multimeric polypeptide molecule in which two or more monomeric subunits are linked together as a single polypeptide ("fusion multimer"). These fusion multimers are more easily and rapidly refolded than unfused multimers, because the reactions necessary to generate the biologically active multimeric form of the polypeptide proceed with first order, rather than second or higher order, reaction kinetics. Fusion multimers also eliminate the simultaneous formation of undesired polypeptide by-products during refolding. The fusion multimers of the present invention specifically include PDGF fusion dimers.
Claims17
A biologically active protein comprising two subunits separated by a spacer moiety and forming a single continuous polypeptide wherein each subunit is a member of the PDGF family and wherein the spacer moiety is selected from the pre-pro region of a PDGF precursor protein.
A nucleic acid sequence encoding a biologically active protein comprising two subunits separated by a spacer moiety and forming a single continuous polypeptide wherein each subunit is a member of the PDGF family and wherein the spacer moiety is selected from the pre-pro region of a PDGF precursor protein.
A transfected host cell containing a nucleic acid sequence encoding a biologically active protein comprising two subunits separated by a spacer moiety and forming a single continuous polypeptide wherein each subunit is a member of the PDGF family and wherein the spacer moiety is selected from the pre-pro region of a PDGF precursor protein.
Dependencies are inferred from the claim text.