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US 5,705,478 AGranted
Covalently linked .beta. subunits of the glycoprotein hormones as antagonists
AssigneeWashington University
InventorIrving Boime
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Abstract
Single-chain forms of the glycoprotein hormone quartet, at least some members of which are found in most vertebrates, are disclosed. The single chain compounds of the invention comprise two .beta. subunits of the glycoprotein hormones, which .beta. subunits are the same or different. These "two-.beta." forms are antagonists of glycoprotein hormone activity.
Claims11
A glycosylated or nonglycosylated single chain glycoprotein hormone antagonist of the formula
- .beta.-(linker).sub.n -.beta.
- wherein each .beta. is independently selected from the group consisting of the glycoprotein hormone .beta. subunits FSH, TSH, LH and CG, n is 0 or 1, and "linker" is a linker moiety;
- and wherein:
- the amino acid sequence of said first .beta.-subunit is linked covalently through a C-terminal carboxyl or N-terminal amino group, optionally through said linker moiety, to a C-terminal carboxyl or N-terminal amino group of the amino acid sequence of said second .beta.-subunit,
- wherein said .beta.-subunits consist of the native amino acid sequences of said hormones or variants of said amino acid sequences.
2 more elements
Dependencies are inferred from the claim text.