Back to results
US 5,705,398 AGranted
Methods for identifying inhibitors of LPS-mediated LBP binding
AssigneeThe Scripps Research Institute
InventorsDouglas N. Mintz, Peter Tobias, Richard Ulevitch
Life of this patent
Estimating the term…
Abstract
The present invention describes a rapid method for the identification of compounds/reagents which inhibit lipopolysaccharide (LPS) binding to LPS binding protein (LBP), and kits for practicing the method.
Claims12
A method for identifying a compound which inhibits lipopolysaccharide (LPS) binding to LPS binding protein (LBP), which method comprises:
- a) measuring the fluorescence emitted by a first binding reaction admixture in solution that comprises:
- i) fluoresceinated LPS, and
- ii) isolated LBP;
- b) measuring the fluorescence emitted by a second binding reaction admixture in solution that comprises:
- i) fluoresceinated LPS,
- ii) isolated LBP, and
- iii) said compound;
- and
- c) identifying a reduction in measured fluorescence emitted by said second binding reaction admixtures compared to said first binding reaction admixture, thereby identifying said inhibition activity of said compound.
6 more elements
A method for identifying a compound which inhibits lipopolysaccharide binding protein (LBP)-dependent binding of lipopolysaccharide (LPS) to monocyte receptor CD14, which method comprises:
- a) measuring the fluorescence emitted by a first binding reaction admixture in solution that comprises:
- i) fluoresceinated LPS,
- ii) isolated LBP, and
- iii) isolated CD14;
- b) measuring the fluorescence emitted by a second binding reaction admixture in solution that comprises:
- i) fluoresceinated LPS,
- ii) isolated LBP,
- iii) isolated CD14, and
- iv) said compound;
- and
- c) identifying a reduction in measured fluorescence emitted by said second binding reaction admixture compared to said first binding reaction admixture, thereby identifying said inhibition activity of said compound.
8 more elements
Dependencies are inferred from the claim text.