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US 5,705,390 AGranted
Bioreactor
AssigneeInterpharm Laboratories Ltd.
InventorsAvinoam Kadouri, Yehoshua Aloni, Mordechai Geron
Life of this patent
Estimating the term…
Abstract
A radial flow bioreactor comprising a reaction vessel having an inlet and an outlet respectively for adding and withdrawing a culture medium and at least one cell basket within the reaction vessel for accommodating a plurality of cell carriers and having side wall perforations so dimensioned as to prevent passage of the cell carriers therethrough.
Claims9
A radial flow bioreactor for cell propagation, comprising:
- a reaction vessel having an inlet and an outlet for adding and withdrawing, respectively, culture medium;
- at least one cell basket within the reaction vessel for accommodating a plurality of cell carriers therein with associated cells to be propagated, each of said at least one cell basket having
- a base member;
- a roof member;
- an outer side wall, having perforations therein, wherein said base and roof members support said outer side wall around the respective outer peripheries thereof, said outer side wall having a longitudinal axis;
- and
- an inner cylinder having a side wall with perforations, supported between said base and roof members and having a longitudinal axis coaxial with the longitudinal axis of said outer side wall, whereby a cavity is defined by said outer side wall and said side wall of said cylinder;
- wherein said base and roof members each have an aperture therein at the appropriate ends of said inner cylinder so as to allow culture medium to flow into the inner cylinder from both ends thereof;
- and
- circulation means for urging the culture medium through the apertures in said base and roof members of each said at least one cell basket, through the perforation in said side wall of said inner cylinder thereof, through the cavity thereof, and thence radially through the perforations of said outer side wall thereof, said circulation being of sufficiently low velocity so as not to create shear forces within each said cavity great enough to cause the cells to be destroyed or removed from their carriers when in use, said circulation means comprising a system of turbine blades coupled to the circumference of each said at least one cell basket so as to rotate therewith and thereby augment the radial circulation of the culture medium from the longitudinal axis through the cavity of each said at least on cell basket, and rotating means for rotating each of said at least one cell basket about the longitudinal axis thereof.
7 more elements
A cell basket comprising:
- a base member;
- a roof member;
- an outer side wall, having perforations therein, wherein said base and roof members support said outer side wall around the respective outer peripheries thereof, said outer side wall having a longitudinal axis;
- an inner cylinder having a side wall with perforations, supported between said base and roof members and having a longitudinal axis substantially parallel to the longitudinal axis of said outer side wall, whereby a cavity is defined by said outer side wall and said side wall of said cylinder, wherein said base and roof members each have an aperture therein at the appropriate ends of said inner cylinder so as to allow culture medium to flow into the inner cylinder from both ends thereof;
- and
- a system of turbine blades coupled to the circumference of said outer side wall in such a manner that when the cell basket is caused to rotate around the longitudinal axis thereof in a liquid medium the turbine blades will augment the flow of the culture medium through said base and roof members into said inner cylinder and radially through the perforations of said inner cylinder side wall, said cavity, and the perforations of said outer side wall.
3 more elements
Dependencies are inferred from the claim text.