Nucleotide sequences encoding a thermostable alkaline protease
AssigneeCornell Research Foundation, Inc.
InventorsDavid B. Wilson, Guifang Lao
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Abstract
Nucleotide sequences, derived from a thermophilic actinomycete microorganism, which encode a thermostable alkaline protease are disclosed. Also disclosed are variants of the nucleotide sequences which encode a polypeptide having thermostable alkaline proteolytic activity. Recombinant thermostable alkaline protease or recombinant polypeptide may be obtained by culturing in a medium a host cell genetically engineered to contain and express a nucleotide sequence according to the present invention, and recovering the recombinant thermostable alkaline protease or recombinant polypeptide from the culture medium.
Claims15
An isolated nucleic acid molecule comprising a nucleotide sequence, wherein the nucleotide sequence is selected from the group consisting of:
- (a) SEQ ID NO:1 encoding a thermostable alkaline protease,
- (b) a portion of SEQ ID NO:1 which is at least 17 bp and specifically hybridizes to SEQ ID NO:1, and
- (c) a variant sequence of SEQ ID NO:1 encoding a polypeptide having thermostable alkaline protease activity, wherein said variant sequence has a sequence identity of greater than about 85% with SEQ ID NO:1 or with the open reading frame of SEQ ID NO:1 beginning at nucleotide 327 and ending at nucleotide 1451 of SEQ ID NO:1.
Dependencies are inferred from the claim text.