US 5,705,371 A · Bacterial strain of escherichia coli BKIIM B-3996 as the producer of L-threonine

Bacterial strain of escherichia coli BKIIM B-3996 as the producer of L-threonine

AssigneeAjinomoto Co., Inc.

InventorsVladimir Georgievich Debabov, Jury Ivancvich Kozlov, Evgeny Moiseevich Khurges, Vitaly Arkadievich Livshits, Nelli Isaakcvna Zhdanova, Mikhail Markovich Gusyatiner, Alexandr Konstantinovich Sokolov, Tatyana Alexandrovna Bachina, Nikolai Kazimirovich Yankovsky, Jury Dmitrievich Tsygankov, Andrei Jurievich Chistoserdov, Tatyana Grigorievna Plotnikova, Irina Clegovna Shakalis, Alla Valentinovna Belareva, Raisa Alexandrovna Arsatiants, Albert Fedorovich Sholin, Tamara Mikhailcvna Pozdnyakova

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Abstract

A bacterial strain of Escherichia coli BKIIM B-3996, a producer of L-threonine, containing a recombinant plasmid pVIC40 and deposited on Nov. 19, 1987 in the collection of microorganism cultures at the USSR Antibiotics Research Institute under Reg. No. 1867.

Claims20

Dependent claims

An E. coli strain which produces L-threonine, derived by a process comprising the steps of:

(i) transducing E. coli strain M1 with a first bacteriophage P1 bearing genetic material necessary for saccharose assimilation, and
(ii) isolating spontaneously occuring, threonine resistant a transductant capable of utilizing saccharose or a saccharose-bearing substrate as a source of carbon.

Dependencies are inferred from the claim text.

Facts

Application

08/633,028Filed 16 Apr 1996 · continuation

Priority

12 Jun 1990

Granted

6 Jan 1998

Term ends

Not estimatedThe source records don’t support an estimate.

Classification4 codes

C12P 13/08
Alpha- or beta- amino acids: lysine; Diaminopimelic acid; Threonine; Valine
C12N 15/70
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression: vectors or expression systems…
C12P
Fermentation or enzyme-using processes to synthesise a desired chemical compound or composition
C12N
Microorganisms or enzymes

Family

Serial499,278Parent of this division · filed 12 Jun 1990

Serial872,141Parent of this continuation · filed 22 Apr 1992

US 5,175,107 AParent of this division · filed 29 Dec 1992

Serial336,563Parent of this continuation · filed 9 Nov 1994

Serial430,455Parent of this continuation · filed 28 Apr 1995

US 5,538,873 AParent of this continuation · filed 23 Jul 1996

US 5,631,157 AParent of this continuation · filed 20 May 1997

US 5,705,371 AThis patent

From the US filing history. Foreign family members aren’t listed.