Process for producing L-amino acids by fermentation
AssigneeAjinomoto Co., Inc.
InventorsTakayasu Tsuchida, Haruo Uchibori, Hiroshi Takeuchi, Mitsuyoshi Seki
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Abstract
Culturing an L-amino acid producing microorganism belonging to the genus Brevibacterium or Corynebacterium and having a resistance to a peptide containing glutamic acid or aspartic acid gives L-amino acids in high yield.
Claims4
A process for producing an L-amino acid, which comprises culturing a mutant L-amino acid producing microorganism belonging to a genus selected from Brevibacterium and Corynebacterium which is resistant to a dipeptide at a concentration of 0.3% in a liquid medium for a time sufficient to produce said L-amino acid;
- and
- recovering said L-amino acid produced wherein
- 1) L-glutamine is produced from a tyr-glu resistant mutant of Brevibacterium flavum ATCC 14067 or an ala-glu resistant mutant of Corynebacterium acetoacidophilum ATCC 13870;
- 2) L-lysine is produced from a val-glu resistant mutant of Brevibacterium lactofermentum ATCC 13869 or an ala-glu resistant mutant of Corynebacterium glutamicum ATCC 13032;
- 3) L-arginine is produced from a tyr-glu resistant mutant of Brevibacterium flavum ATCC 14067;
- 4) L-glutamic acid is produced from a tyr-glu resistant mutant of Brevibacterium lactofermentum ATCC 13869 or an ala-glu resistant mutant of Corynebacterium glutamicum ATCC 13032;
- 5) L-histidine is produced from a trp-glu resistant mutant of Brevibacterium flavum ATCC 14067 or a glu-his resistant mutant of Corynebacterium glutamicum ATCC 13032;
- 6) L-proline is produced by a tyr-glu resistant mutant of Brevibacterium flavum ATCC 14067;
- and
- 7) L-isoleucine is produced from an ala-asp resistant mutant of Brevibacterium flavum ATCC 14067, and
- wherein said mutant microorganism is obtained by mutation of a parent strain, and said microorganism produces the L-amino acid in an amount greater than the amount produced by the parent strain.
A process for producing an L-amino acid, which comprises culturing a mutant L-amino acid producing microorganism belonging to the genus Brevibacterium which is resistant to a dipeptide at a concentration of 0.3% in a liquid medium for a time sufficient to produce said L-amino acid;
- and
- recovering said L-amino acid produced, wherein the amino acid is L-glutamine, the mutant microorganism is a tyr-glu resistant mutant of Brevibacterium flavum ATCC 14067, the mutant microorganism is obtained by mutation of a parent strain, and the mutant microorganism produces the L-amino acid in an amount greater than the amount produced by the parent strain.
Dependencies are inferred from the claim text.