Modified signal sequences
AssigneeGist-Brocades N.V.
InventorsAlfonsusJohannes Bonekamp, Marcellis Wilhelmus Elisabeth Maria Van Tilborg
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Abstract
The present invention describes a method for reducing processing ambiguity of signal peptidases. Specifically, the C-terminus of a signal sequence is mutated in a such a manner that the secreted protein contains the correct N-terminal amino acid. Furthermore, the present invention provides an hIL-3 which is essentially homogenous at its N-terminus.
Claims18
A nucleic acid molecule that comprises a nucleotide sequence encoding a fusion protein of the formula
- signal peptide-mature protein
- wherein said signal peptide has an amino acid sequence modified from that of a corresponding wild-type signal peptide which contains Ala at positions -1 to -4 so that the codon at position -4 has been modified to encode Ser or Gly whereby a signal peptidase cleavage site is removed by said modification.
A recombinant host cell comprising an expression system for a nucleotide sequence encoding a precursor protein of the formula:
- signal peptide-mature protein
- wherein the signal peptide-encoding nucleotide sequence has been modified from a wild-type signal peptide which contains Ala at positions -1 to -4 so that the codon at position -4 has been modified to encode Ser or Gly so as to reduce amino terminal heterogeneity in said mature protein produced and secreted by said cells.
Dependencies are inferred from the claim text.