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US 5,705,361 AGranted
Hybrid transposon with PT operon, plasmids and bacterial strains therefor
AssigneeGesellschaft fur Biotechnologische Forschung mbH (GBF)
InventorsMark Walker, Kenneth Timmis
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Abstract
The invention relates to a hybrid transposon having a PT operon, to plasmids and to bacterial strains (hosts) therefor.
Claims12
A plasmid for the stable expression of pertussis toxin in a bacterial microorganism which comprises;
- a promoterless hybrid transposon wherein a transposase gene is arranged outside of inverted repeats of the transposon;
- and
- having a pertussis toxin operon inserted between the inverted repeats;
- said inverted repeats being in shortened form, thereby permitting a further transposition;
- said plasmid permitting a stable insertion of the pertussis toxin operon into a bvg-negative bacterial strain.
2 more elements
A plasmid which is promotorless having the following features and is
- a suicide vector that has a mini-transposon for pertussis toxin expression for transcriptional fusions with native promoters, having
- 1. a mob site,
- 2. an ampicillin resistance gene and
- 3. in each case, 19 base pairs of the terminal Tn5 end which flank,
- 4. a kanamycin resistance gene and
- 5. a promotorless operon;
- and also having
- 6. a IS50.sub.R -transposase gene.
5 more elements
Dependencies are inferred from the claim text.