Hirudin, pharmaceutical compositions comprising it and their use
AssigneeTransgene S.A.
InventorsPaul Tolstoshev, Richard Harvey, Michael Courtney, Jean-Pierre Lecocq
Life of this patent
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Abstract
The present invention relates to vectors for cloning, in a host cell, hirudin or an analog of hirudin, characterized in that it comprises the gene for encoding hirudin or an analog of hirudin, and the elements for the expression of this gene in said host cell, said coding gene beginning, after the starting sequence, by an Ile codon and a Thr codon. This invention also relates to the process for the preparation of hirudin or analogs of hirudin as well as the hirudin or anologs of hirudin obtained.
Claims7
A substantially pure hirudin having an amino acid sequence as follows:
- ______________________________________ 1 5 10 ATT ACT TAC ACT GAT TGT ACA GAA TCG GGT Ile THr Tyr Thr Asp Cys Thr Glu Ser Gly 15 20 CCA AAT TTG TGC CTC TGC GAG GGA AGC AAT Gln Asn Leu Cyc Leu Cyc Glu Gly Ser Asn 25 30 GTT TGC CCT AAA GGC AAT AAG TGC ATA TTG Val Cys Gly Lys Gly Asn Lys Cys Ile Leu 35 40 GGT TCT AAT GGA AAG GGC AAC CAA TGT GTC Gly Ser Asn Gly Lys Gly Asn Gln Cys Val 45 50 ACT GGC GAA GGT ACA CCG AAC CCT GAA AGC Thr Gly Glu Gly Thr Pro Asn Pro Glu Ser 55 60 CAT AAT AAC GGC GAT TTC GAA GAA ATT CCA His Asn Asn Gly Asp Phe Glu Glu Ile Pro 65 GAA GAA TAT TTA CAA Glu Glu Tyr Leu Gln. ______________________________________
A substantially pure variant hirudin having an amino acid sequence as follows:
- __________________________________________________________________________ 1 5 10 ATT ACT TAC ACT GAT TGT ACA GAA TCG GGT CCA AAT TTG TGC Ile Thr Tyr Thr Asp Cys Thr Glu Ser Gly Gln Asn Leu Cys 15 20 25 CTC TGC GAG GGA AGC AAT GTT TGC CCT AAA GGC AAT AAG TGC Leu Cys Glu Gly Ser Asn Val Cys Gly Lys Gly Asn Lys Cys 30 35 40 ATA TTG GGT TCT AAT GGA AAG GGC AAC CAA TGT GTC ACT GGC Ile Leu Gly Ser Asn Gly Lys Gly Asn Gln Cys Val Thr Gly 45 50 55 GAA GGT ACA CCG AAC CCT GAA AGC CAT AAT AAC GGC GAT TTC Glu Gly Thr Pro Asn Pro Glu Ser His Asn Asn Gly Asp Phe 60 65 GAA GAA ATT CCA GAA GAA TAT TTA CAA Glu Glu Ile Pro Glu Glu Tyr Leu Gln __________________________________________________________________________
- wherein the amino acid Ile in position 1 is replaced by the amino acid Val and the amino acid Thr in position 2 is replaced by the amino acid Val.
Dependencies are inferred from the claim text.