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US 5,705,352 AGranted
Multiple drug resistance gene of Aspergillus fumigatus
AssigneeEli Lilly and Company
InventorsRobert B. Peery, Paul L. Skatrud
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Abstract
The invention provides isolated nucleic acid compounds encoding a multiple drug resistance protein of Aspergillus fumigatus. Vectors and transformed host cells comprising the multiple drug resistance-encoding DNA of Aspergillus fumigatus MDR-1 are also provided. The invention further provides assays which utilize these transformed host cells.
Claims20
An isolated nucleic acid molecule that comprises nucleic acid encoding Aspergillus fumigatus MDR-1.
A method for determining the fungal MDR inhibition activity of a compound which comprises:
- a) growing a culture of yeast cells, transformed with a vector which provides expression of the Afu-MDR-1, in the presence of:
- (i) an antifungal agent to which said yeast cell is resistant, but to which said yeast cell is sensitive in its untransformed state;
- (ii) a compound suspected of possessing Aspergillus fumigatus MDR inhibition activity;
- and
- b) determining the fungal MDR inhibition activity of said compound by measuring the ability of the antifungal agent to inhibit the growth of said yeast cell.
2 more elements
Dependencies are inferred from the claim text.