Methods and kits for preparing nucleic acids using cyclodextrin
AssigneeAmersham International plc
InventorsArne Lundin, John George Anson, Michael Kenneth Kenrick
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Abstract
A method of preparing nucleic acids by obtaining an impure nucleic acid preparation, treating said preparation with phenol and adding a cyclodextrin to the treated preparation to neutralize the phenol.
Claims17
In a method of preparing nucleic acids comprising obtaining a sample containing cells and treating said sample with an extractant to lyse said cells and release nucleic acids, the improvement which comprises adding a cyclodextrin to the treated sample to neutralize the extractant.
A kit for nucleic acid manipulation comprising
- an extractant for treating cells to recover nucleic acid therefrom
- a cyclodextrin for neutralizing the extractant
- at least one polymerase or restriction enzyme for further processing the recovered nucleic acid by amplification or enzymatic modification or restriction.
In a method of preparing nucleic acids comprising obtaining an impure nucleic acid preparation and treating said preparation with phenol, the improvement which comprises adding a cyclodextrin to the treated preparation to neutralize the phenol.
Dependencies are inferred from the claim text.