US 5,705,338 AGranted

Reduction of background in noncompetitive binding assays

AssigneeChiron Diagnostics Corporation

InventorsUri Piran, Laurie Ann Livshin, Richard A. Martinelli, William J. Riordan, John T. Unger

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Abstract

Novel non-competitive assay techniques have been developed which not only improve sensitivity, but also are convenient and less susceptible to interfering factors. They are compatible with existing instruments and can be run in one or more test tubes. The analyte is reacted with labeled specific binder, after which the mixture is reacted with (1) an insoluble material attached to an analyte derivative and (2) a solid phase carrying a binder. The solid phase is then separated, and the label attached to the solid phase is measured. Variations of the procedure include the use of a reversible bridge for attaching the insoluble material to the analyte mimic and the conduct of the assay in various porous media, such as paper, chromatographic and electrophoretic media, and dipsticks.

Claims7

Dependent claims

A binding assay procedure for determination of an analyte, such procedure being conducted in one container, by

a. mixing the solution containing the analyte with a labeled specific binder which binds to a first binding site on the analyte to form an analyte-labeled specific binder complex,
b. contacting the solution from step a with one or more materials which comprise
1. a reagent which contains an insoluble material which is attached by a reversible bridge to an analyte derivative or an analyte mimic that binds to the labeled specific binder that had not bound analyte to form an insoluble material-labeled specific binder complex and
2. a reagent that contains a second binder which binds to one portion of the analyte-labeled specific binder complex, such second binder being attached to a solid phase,
such that the insoluble material, by binding to the labeled specific binder that had not bound analyte, inhibits the binding of the unreacted labeled specific binder to the solid phase,
c. separating the solid phase from the insoluble material and the liquid components of the reaction mixture and washing the solid phase with a solution containing one or more materials that disrupt the reversible bridge between the label and any insoluble material remaining in the container with said solid phase,
d. measuring the label associated with the solid phase, and
e. determining the amount of analyte by comparing the amount of said label measured to that in a reference solution.

5 more elements

Dependencies are inferred from the claim text.

Facts

Application: 08/434,743Filed 4 May 1995 · continuation in part
Priority: 15 Sep 1993
Granted: 6 Jan 1998
Term ends: Not estimatedThe source records don’t support an estimate.

Classification6 codes

C12Q 1/6813
Involving nucleic acids: hybridisation assays
C12Q 1/6834
Hybridisation assays: enzymatic or biochemical coupling of nucleic acids to a solid phase
G01N 33/54306
With an insoluble carrier for immobilising immunochemicals: solid-phase reaction mechanisms
G01N 33/008
Testing material properties on manufactured objects: sport articles, e.g. balls, skis or rackets

Family

Serial121,806Parent of this continuation-in-part · filed 15 Sep 1993
US 5,445,936 AParent of this continuation-in-part
US 5,705,338 AThis patent

From the US filing history. Foreign family members aren’t listed.

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