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US 5,705,330 AGranted
Chemiluminescent immunoassay for antibody detection
AssigneeAbbott Laboratories
InventorsDinesh O. Shah, Russell B. Richerson
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Abstract
A chemiluminescent assay utilizing a precomplex reagent of a probe comprising an enhancer compound such as a hapten and a conjugate comprising a chemiluminescent signal generating compound. Kits for performing such an chemiluminescent assay are also provided.
Claims9
A method for determining the presence of a Hepatitis C Virus (HCV) analyte in a test sample by specific amplification of a chemiluminescent signal generated from a heterogeneous immunoassay, comprising:
- a. incubating a test sample containing a HCV analyte with an analyte-specific binding pair member for a time and under conditions sufficient to form analyte/analyte specific binding member pair complexes;
- b. contacting the analyte/analyte specific binding member pair complexes with a precomplex wherein said precomplex comprises 1) a probe comprising an enhancer compound attached to an analyte-specific binding member and 2) a conjugate comprising a chemiluminescent signal generating compound attached to an enhancer-specific binding member, and incubating said second mixture for a time and under conditions sufficient to form analyte/analyte specific binding member pair/precomplex complexes
- wherein said chemiluminescent signal generating compound is an acridinium compound or a derivative thereof;
- c. separating said resulting analyte/analyte specific binding member pair/precomplex complexes of step b from free, unbound precomplexes;
- and
- d. determining the presence of the HCV analyte in the test sample by measuring the detectable signal.
3 more elements
A kit for detecting an HCV analyte, comprising:
- an HCV antigen;
- and
- a single container containing a precomplex reagent wherein said precomplex reagent comprises 1) a probe which comprises an enhancer compound and 2) a conjugate which comprises a chemiluminescent signal generating compound, wherein said chemiluminescent signal generating compound is an acridinium or a derivative thereof.
Dependencies are inferred from the claim text.