Glycolipid enzyme-polymer conjugates
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorsRobert G. L. Shorr, Carl W. Gilbert, Brian M. Martin, Myung-Ok Cho, Edward J. Ginns
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Abstract
Conjugates containing glucocerebrosidase and non-antigenic polymers such as polyethylene glycol are disclosed. The conjugates circulate for extended times and have prolonged activity in vivo when compared to unmodified enzymes. The conjugates are useful in the treatment of Gaucher's Disease and have improved enzyme activity at the pH ranges associated with lysosomal, arterial and capillary regions.
Claims10
A method of treating Gaucher's Disease comprising administering an effective amount of a glucocerebrosidase conjugate having enhanced enzymatic activity at pH ranges of from about 4.0-5.0 and about 6.5-7.5, comprising recombinant glucocerebrosidase and from about 1 to about 25 poly(alkylene oxide) strands, each strand having a molecular weight of from about 1,000 to about 15,000, and each strand being covalently linked via a urethane linkage to an amino group on said recombinant glucocerebrosidase.
Dependencies are inferred from the claim text.