Antigen specific plasmacytomas and antibodies derived therefrom
AssigneesThe United States of America as represented by the Department of Health and Human Services, Wisconsin Alumni Research Foundation
InventorsRex G. Risser, deceased, David A. Largaespada, Joseph F. Mushinski, Eva M. Weissinger, Harald Mishak
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Abstract
Disclosed herein is a method for producing antibodies against an antigen of interest. Animal cells are exposed to both the antigen of interest and a recombinant retroviral vector. The vector contains a combination of oncogenes capable of inducing plasmacytomas. Plasmacytoma formation takes place rapidly and takes place in the presence of the antigen. A very high proportion of the plasmacytomas that are recovered are antigen specific.
Claims8
A method for production of antibodies against a specified antigen, the method comprising:
- immunizing a first murine host with an antigen to create immune responding B cells in the host;
- removing the immune responding B cells from the first murine host;
- transforming the immune responding B cells in vitro by introducing a vector comprising a myc oncogene and a vector comprising an abl oncogene into the cells;
- and
- injecting the transformed cells into a second murine host in which the transformed cells can become established to form a plasmacytoma that produces antibodies against said antigen.
Dependencies are inferred from the claim text.