Stealth virus nucleic acids and related methods
OwnerWilliam John Martininventor
Life of this patent
Estimating the term…
Abstract
A method of detecting a stealth virus is provided by culturing a sample under conditions in which any stealth virus in the sample is able to induce a cytopathic effect. A method for culturing a virus is also provided by (a) cocentrifuging a sample of said virus with a permissive cell line of indicator cells; (b) inoculating the cell mixture into culture vessels; (c) adding viral enhancing medium to the culture; and (d) detecting in vitro a cytopathic effect in the permissive cell line.
Claims1
An isolated, purified or enriched stealth virus nucleic acid molecule corresponding to at least a portion of a stealth virus nucleic acid sequence in the MRC-5 cell line, ATCC accession number VR2343, obtained by a method comprising:
- (a) amplification of nucleic acid sequences from a virus infected culture of cells by low stringency polymerase chain reaction (PCR) to produce PCR amplified nucleic acid sequences, and;
- (b) isolation of said PCR amplified nucleic acid sequences.
Dependencies are inferred from the claim text.