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US 5,703,218 AGranted
Oligonucleotides containing hydroxyl-protecting groups orthogonally removable by reduction
AssigneeChiron Corporation
InventorsMichael S. Urdea, Thomas Horn
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Abstract
Hydroxyl-protecting groups orthogonally removable by reduction with a liquid reducing agent are disclosed. The novel hydroxyl-protecting groups are particularly useful in the chemical synthesis of linear and branched oligonucleotide structures, as they are readily removed from the protected molecule with mild reagents such as dithionite. Examples of such hydroxyl-protecting groups include the 2-methylene-9,10-anthraquinone (Maq) carbonate ester and the p-nitrobenzyl carbonate ester.
Claims5
An oligonucleotide chain in which from one to all of the nucleotide residues have the structural formula ##STR8## wherein:
- R is selected from the group consisting of ##STR9## R' is hydrogen or phenyl;
- R.sup.3 is selected from the group consisting of hydrogen, methyl, I, Br and F;
- R.sup.4 is hydrogen or methyl;
- and
- Z is selected from the group consisting of ##STR10## wherein x and y may be the same or different and are integers in the range of 1 to 8 inclusive.
2 more elements
Dependencies are inferred from the claim text.