Nucleotide fragment of the 23S ribosomal RNA of mycobacteria, derived probes and primers, reagent and detection method

A probe for the identification of one or two species of bacteria of the genus Mycobacterium, said probe comprising, as its sole nucleotide sequence, a first sequence of no more than one hundred modified or unmodified nucleotides, said first sequence comprising a second sequence selected from the group consisting of the following sequences:

• beginning at nucleotide No. 10 and ending at nucleotide No. 24 of at least one of sequences SEQ ID NO 1 to 14,
• beginning at nucleotide No. 26 and ending at nucleotide No. 45 of at least one of sequences SEQ ID NO 1 to 14,
• beginning at nucleotide No. 1 and ending at nucleotide No. 20 of at least one of sequences SEQ ID NO 15 to 28,
• beginning at nucleotide No. 26 and ending at nucleotide No. 43 of at least one of sequences SEQ ID NO 15 to 28,
• beginning at nucleotide No. 4 and ending at nucleotide No. 22 of at least one of sequences SEQ ID NO 29 to 41,
• beginning at nucleotide No. 12 and ending at nucleotide No. 29 of at least one of sequences SEQ ID NO 42 to 53,
• beginning at nucleotide No. 36 and ending at nucleotide No. 56 of at least one of sequences SEQ ID NO 42 to 53,
• beginning at nucleotide No. 13 and ending at nucleotide No. 32 of at least one of sequences SEQ ID NO 54 to 67,
• beginning at nucleotide No. 5 and ending at nucleotide No. 23 of at least one of sequences SEQ ID NO 68 to 79,
• beginning at nucleotide No. 15 and ending at nucleotide No. 33 of at least one of sequences SEQ ID NO 80 to 93,
• beginning at nucleotide No. 1 and ending at nucleotide No. 21 of at least one of sequences SEQ ID NO 94 to 106 and SEQ ID NO 166 to 178,
• beginning at nucleotide No. 44 and ending at nucleotide No. 76 of at least one of sequences SEQ ID NO 94 to 106 and SEQ ID NO 166 to 178,
• beginning at nucleotide No. 67 and ending at nucleotide No. 85 of at least one of sequences SEQ ID NO 94 to 106 and SEQ ID NO 166 to 178,
• beginning at nucleotide No. 6 and ending at nucleotide No. 26 of at least one of sequences SEQ ID NO 107 to 120,
• beginning at nucleotide No. 1 and ending at nucleotide No. 18 of at least one of sequences SEQ ID NO 121 to 136,
• beginning at nucleotide No. 39 and ending at nucleotide No. 55 of at least one of sequences SEQ ID NO 121 to 136,
• beginning at nucleotide No. 11 and ending at nucleotide No. 26 of at least one of sequences SEQ ID NO 139 to 144, SEQ ID NO 146, SEQ ID NO 147 and SEQ ID NO 149 to 152,
• beginning at nucleotide No. 5 and ending at nucleotide No. 22 of at least one of sequences SEQ ID NO 153 to 165,
• their corresponding DNA sequences, and sequences fully complementary to said RNA and DNA sequences, wherein said probe selectively hybridizes to RNA or DNA of only one or two species of the genus Mycobacterium.

A probe comprising, as its sole nucleotide sequence, a probe sequence of no more than one hundred modified or unmodified nucleotides, said probe sequence comprising a nucleotide sequence selected from the group consisting of the following sequences of a 23S RNA of a species of the genus Mycobacterium:

• beginning at nucleotide No. 137 and ending at nucleotide No. 179,
• beginning at nucleotide No. 206 and ending at nucleotide No. 248,
• beginning at nucleotide No. 269 and ending at nucleotide No. 289,
• beginning at nucleotide No. 289 and ending at nucleotide No. 290,
• beginning at nucleotide No. 341 and ending at nucleotide No. 372,
• beginning at nucleotide No. 645 and ending at nucleotide No. 661,
• beginning at nucleotide No. 1201 and ending at nucleotide No. 1242,
• beginning at nucleotide No. 1409 and ending at nucleotide No. 1420,
• beginning at nucleotide No. 1493 and ending at nucleotide No. 1515,
• beginning at nucleotide No. 1703 and ending at nucleotide No. 1761,
• beginning at nucleotide No. 1854 and ending at nucleotide No. 1876,
• beginning at nucleotide No. 2126 and ending at nucleotide No. 2161 of species other than M. avium,
• their corresponding DNA sequences, and sequences fully complementary to said RNA and DNA sequences, the numbers of the nucleotides corresponding to their position relative to the nucleotide sequence of Escherichia coli 23S ribosomal RNA, wherein said probe selective hybridizes to RNA or DNA of only one or two species of the genus Mycobacterium.

A primer for the specific reverse transcription of a variable region of a 23S ribosomal RNA sequence of mycobacteria into a complementary DNA sequence, said primer consisting of a sequence of no more than one hundred modified or unmodified nucleotides, said sequence comprising a nucleotide sequence of at least eight contiguous nucleotides of at least one of the following nucleotide sequences of a 23S RNA of a species of the genus Mycobacterium:

• beginning at nucleotide No. 137 and ending at nucleotide No. 179,
• beginning at nucleotide No. 206 and ending at nucleotide No. 248,
• beginning at nucleotide No. 269 and ending at nucleotide No. 289,
• beginning at nucleotide No. 289 and ending at nucleotide No. 290,
• beginning at nucleotide No. 341 and ending at nucleotide No. 372,
• beginning at nucleotide No. 645 and ending at nucleotide No. 661,
• beginning at nucleotide No. 1201 and ending at nucleotide No 1242,
• beginning at nucleotide No. 1409 and ending at nucleotide No 1420,
• beginning at nucleotide No. 1493 and ending at nucleotide No 1515,
• beginning at nucleotide No. 1703 and ending at nucleotide No 1761,
• beginning at nucleotide No. 1854 and ending at nucleotide No 1876,
• beginning at nucleotide No. 2126 and ending at nucleotide No 2161 of species other than M. avium,
• their corresponding DNA sequences, and sequences fully complementary to said RNA and DNA sequences, the numbers of the nucleotides corresponding to their position relative to the nucleotide sequence of Escherichia coli 23S ribosomal RNA, wherein said primer selectively hybridizes to RNA or DNA of only one or two species of the genus Mycobacterium.